Ssdp proteins interact with the LIM-domain-binding protein Ldb1 to regulate development
Ssdp proteins interact with the LIM-domain-binding protein Ldb1 to regulate development
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DOI：
10.1073/pnas.212532399
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发表时间：
2002-10-29
影响因子：
11.1
通讯作者：
Westphal, H
中科院分区：
文献类型：
--
作者：
Chen, L;Segal, D;Westphal, H
LIM结构域结合蛋白Ldb1是转录复合物组装的关键因子，该转录复合物涉及LIM同源结构域蛋白和调节动物发育的其他转录因子。我们确定Ssdp蛋白（以前描述为序列特异性，单链DNA结合蛋白）作为HeLa细胞中Ldb1相关核复合物的组成部分。Ssdp蛋白与Ldb1在各种其他哺乳动物细胞类型。这种关联是特异性的，不依赖于核酸的存在，并且在功能上具有重要意义。编码Ssdp蛋白的基因在从果蝇到人类的进化过程中非常保守。尽管脊椎动物Ssdp基因家族有几个密切相关的成员，但果蝇Ssdp基因是独特的。在非洲爪蟾中，由果蝇Ssdp或小鼠Ssdp1 mRNA编码的Ssdp与LIM同源框基因Xlim 1一起增强Ldb 1的轴诱导。此外，我们能够证明Ssdp和芯片（Ldb1的苍蝇同系物）在果蝇翅膀发育之间的相互作用。这些研究结果表明，在无脊椎动物和脊椎动物的发展过程中，Ssdp作为Ldb1的辅因子的功能保护。
The LIM-domain-binding protein Ldb1 is a key factor in the assembly of transcriptional complexes involving LIM-homeodomain proteins and other transcription factors that regulate animal development. We identified Ssdp proteins (previously described as sequence-specific, sing le-stranded-DNA-binding proteins) as components of Ldb1-associated nuclear complexes in HeLa cells. Ssdp proteins are associated with Ldb1 in a variety of additional mammalian cell types. This association is specific, does not depend on the presence of nucleic acids, and is functionally significant. Genes encoding Ssdp proteins are well conserved in evolution from Drosophila to humans. Whereas the vertebrate Ssdp gene family has several closely related members, the Drosophila Ssdp gene is unique. In Xenopus, Ssdp encoded by Drosophila Ssdp or mouse Ssdp1 mRNA enhances axis induction by Ldb1 in conjunction with the LIM-homeobox gene Xlim1. Furthermore, we were able to demonstrate an interaction between Ssdp and Chip (the fly homolog of Ldb1) in Drosophila wing development. These findings indicate functional conservation of Ssdp as a cofactor of Ldb1 during invertebrate and vertebrate development.