ESCHERICHIA-COLI MUTANTS ACCUMULATING THE PRECURSOR OF A SECRETED PROTEIN IN THE CYTOPLASM
ESCHERICHIA-COLI MUTANTS ACCUMULATING THE PRECURSOR OF A SECRETED PROTEIN IN THE CYTOPLASM
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DOI：
10.1038/277538a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
BECKWITH, J
中科院分区：
文献类型：
--
作者：
BASSFORD, P;BECKWITH, J
E.大肠杆菌分泌到细胞的外周质区室中。描述了用于分离不能输出该蛋白的突变体的选择程序。这些突变体可能是由于氨基末端信号序列的改变，导致产生的麦芽糖结合蛋白以其前体形式积累在细胞质中。
The maltose binding protein of E. coli is secreted into the external periplasmic compartment of the cell. A selection procedure for the isolation of mutants which fail to export this protein is described. These mutants probably result from alterations in the amino terminal signal sequence, causing the maltose binding protein produced to accumulate in the cytoplasm in its precursor form.