Bead assembly magnetorotation as a signal transduction method for protein detection.
Bead assembly magnetorotation as a signal transduction method for protein detection.
复制标题
珠组装磁旋转作为蛋白质检测的信号转导方法。
DOI：
10.1016/j.bios.2013.03.073
复制
发表时间：
2013
影响因子：
12.6
通讯作者：
Kopelman,Raoul
中科院分区：
文献类型：
--
作者：
Hecht,Ariel;Commiskey,Patrick;Shah,Nicholas;Kopelman,Raoul
本文展示了一种新的蛋白质检测信号转导方法的原理证明，这种方法被称为蛋白头组装磁旋转（BAM）。BAM的原理是利用目标蛋白介导适配体包被的1μm磁珠在1μL的悬浮液滴底部形成磁珠组装体。这种聚头的大小、形状和分形维数都取决于蛋白质的浓度。蛋白质浓度的测量有两种方法：一种是磁旋，其中组装的旋转周期与蛋白质浓度相关；另一种是分形分析。此外，介绍了一种无需显微镜的磁旋检测方法，该方法基于由标准实验室组件构建的简单激光装置。在本文中，我们选择关注凝血酶蛋白，这是该领域原理验证工作的热门选择。
This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1μm magnetic beads into a bead assembly, formed at the bottom of a 1μL hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in which the rotational period of the assembly correlates with the protein concentration, or by fractal analysis. Additionally, a microscope-free magnetorotation detection method is introduced, based on a simple laser apparatus built from standard laboratory components. In this paper, we chose to focus on the protein thrombin, a popular choice for proof-of-principle work in this field.