Titration without Mixing or Dilution: Sequential Injection of Chemical Sensing Membranes.
Titration without Mixing or Dilution: Sequential Injection of Chemical Sensing Membranes.
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无需混合或稀释的滴定：化学传感膜的顺序注入。
DOI：
10.1021/ac960779o
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发表时间：
1997
影响因子：
7.4
通讯作者：
J. Ruzicka
中科院分区：
文献类型：
--
作者：
D. Holman;G. Christian;J. Ruzicka
提出了一种新型的、小型化的滴定方法，即以35 μm的微珠作为半固体滴定剂，保留在非水溶液中。具有内部结合的pH指示剂的琼脂糖珠充当用NaOH水溶液滴定剂溶胀的pH传感膜材料。在用1-丁醇样品中的H（2）SO（4）灌注期间，指示剂监测琼脂糖珠内的剩余滴定剂。通过在流动池中自动包装和处置，使2 mg珠层的不可逆反应成为可能。这种策略取代了膜的优势，在层流条件下的混合和不必要的稀释的负担。琼脂糖环境用NaCl调节以耐受样品中溶解的盐。透过FEP PTFE光学窗口通过光纤进行透射率测量。终点体积和酸浓度之间存在简单的反比关系，因此校准曲线呈线性，R（2）= 0.9980。
A novel, miniaturized titration was developed using beads 35 μm in diameter as semisolid aqueous titrant retained in a nonaqueous sample stream. Agarose beads with internally bound pH indicator served as a pH sensing membrane material swollen with aqueous NaOH titrant. The indicator monitored the remaining titrant within the agarose beads during perfusion with H(2)SO(4) in 1-butanol samples. Irreversible reaction of 2 mg bead layers was made possible by automated packing and disposal in a flow cell. This strategy substituted membrane advantages for the burdens of mixing and unnecessary dilution under laminar flow conditions. The agarose environment was conditioned with NaCl to tolerate dissolved salt in the sample. Transmittance measurements were made via fiber optics through FEP PTFE optical windows. A simple inverse relationship held between endpoint volume and acid concentration so that calibration curves were linear, R(2) = 0.9980.