Control of Subgrain Formation in Protein Crystals by the Application of an External Electric Field,
Control of Subgrain Formation in Protein Crystals by the Application of an External Electric Field,
复制标题
通过施加外部电场控制蛋白质晶体中的亚晶形成，
DOI：
10.1021/cg500946b
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发表时间：
2014
影响因子：
3.8
通讯作者：
and J. Nozawa
中科院分区：
文献类型：
--
作者：
H. Koizumi;S. Uda;K. Fujiwara;M. Tachibana;K. Kojima;and J. Nozawa
X-射线衍射摇摆曲线测量进行了四季母鸡卵白色（HEW）溶菌酶晶体生长与不施加外部交流（AC）电场。摇摆曲线的半高宽分析表明，亚晶粒间的取向差，|θ - φ|和晶体中的局部应变ε导致实验测定的四氢HEW溶菌酶晶体的摇摆曲线变宽。特别是，数据表明，在tetrahydroxyHEW溶菌酶晶体中的缺陷主要是由亚晶粒之间的取向不良引起的。此外，它被确定，在质量的四氢HEW溶菌酶晶体的改进，如通过降低亚晶粒之间的取向差，可以通过在晶体生长期间施加1 MHz的电场来实现。本文还讨论了亚晶取向差的来源，根据杂质进入蛋白质晶体。
X-ray diffraction rocking-curve measurements were performed on tetragonal hen egg white (HEW) lysozyme crystals grown with and without the application of an external alternating current (AC) electric field. Analysis of the full width at half-maximum (fwhm) of the rocking curves indicated that misorientation between subgrains, |θ – φ|, and local strain, ⟨ε⟩, in the crystals contributed to the broadening of the experimentally determined rocking curves for the tetragonal HEW lysozyme crystals. In particular, the data suggested that imperfections in tetragonal HEW lysozyme crystals are predominantly caused by misorientation between subgrains. Moreover, it was determined that improvements in the quality of tetragonal HEW lysozyme crystals, as assessed by a decrease in the misorientation between subgrains, can be achieved by imposing a 1 MHz electric field during crystal growth. This paper also discusses the origin of subgrain misorientation in light of the incorporation of impurities into protein crystals.