In vitro activity of amiloride combined with tobramycin against Pseudomonas isolates from patients with cystic fibrosis.
In vitro activity of amiloride combined with tobramycin against Pseudomonas isolates from patients with cystic fibrosis.
复制标题
阿米洛利联合妥布霉素对囊性纤维化患者分离的假单胞菌的体外活性。
DOI：
10.1128/aac.32.3.395
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发表时间：
1988
影响因子：
4.9
通讯作者：
Aronoff,SC
中科院分区：
文献类型：
--
作者：
Cohn,RC;Jacobs,M;Aronoff,SC
利尿剂阿米洛利作为儿童囊性纤维化（CF）肺部疾病的辅助治疗已在最近的研究中。在初步研究中，该制剂单独使用或与β -内酰胺制剂联合使用对铜绿假单胞菌和洋葱假单胞菌的抑菌活性较差；然而，阿米洛利与妥布霉素对洋葱球菌有明显的协同作用。本研究的目的是确定阿米洛利-妥布霉素对CF呼吸道分离的铜绿假单胞菌、洋葱假单胞菌和嗜麦芽假单胞菌的协同作用程度。琼脂稀释法测定妥布霉素和阿米洛利单独对假单胞菌试验菌株的mic。通过将阿米洛利的四种亚抑制浓度（至少低于MIC的四倍）与妥布霉素的两倍稀释度联合使用，并比较妥布霉素单独使用和联合使用对每种菌株的MIC，来确定协同作用。在测试的最高浓度下，联合用药协同抑制了50%的洋葱芽孢杆菌菌株；该组合对少量铜绿假单胞菌和嗜麦芽假单胞菌具有协同作用。妥布霉素联合阿米洛利在可达到的气道浓度下抑制洋葱球菌。我们得出结论，妥布霉素和阿米洛利联合用药可能对CF患儿的洋葱芽孢杆菌感染有潜在的治疗作用。
The diuretic amiloride has been under recent investigation as adjunctive therapy for pulmonary disease in children with cystic fibrosis (CF). In preliminary studies, the antimicrobial activity of this agent alone or combined with beta-lactam agents against reference strains of Pseudomonas aeruginosa and P. cepacia was poor; however, amiloride was markedly synergistic with tobramycin against P. cepacia. The purpose of this study was to determine the extent of amiloride-tobramycin synergy against CF respiratory isolates of P. aeruginosa, P. cepacia, and P. maltophilia. The MICs of tobramycin and amiloride alone against the Pseudomonas test strains were determined by agar dilution. Synergy was determined by combining each of four subinhibitory concentrations of amiloride (at least fourfold below the MIC) with doubling dilutions of tobramycin and comparing the MIC of tobramycin alone and in combination for each strain. At the highest concentration tested, the drug combination synergistically inhibited 50% of the P. cepacia strains tested; the combination was synergistic against fewer isolates of P. aeruginosa and P. maltophilia. Only P. cepacia was inhibited by tobramycin combined with amiloride at achievable airway concentrations. We conclude that the combination of tobramycin and amiloride may be potentially useful in the treatment of P. cepacia infections in children with CF.