Social dominance in tilapia is associated with gonadotroph hyperplasia
Social dominance in tilapia is associated with gonadotroph hyperplasia
复制标题
DOI：
10.1016/j.ygcen.2013.04.032
复制
发表时间：
2013-10-01
影响因子：
2.7
通讯作者：
Levavi-Sivan, Berta
中科院分区：
文献类型：
--
作者：
Golan, Matan;Levavi-Sivan, Berta
罗非鱼是世界水产养殖中最重要的鱼类之一，也被广泛用作繁殖和行为研究的模式鱼。在繁殖季节，雄性罗非鱼具有很强的领土意识，并形成产卵坑，在那里占优势的雄性会向可用的雌性求爱并产卵。没有领地意识的雄性繁殖的机会要低得多。利用荧光标记促卵泡激素（FSH）促性腺激素细胞的增强型绿色荧光蛋白（EGFP）转基因罗非鱼，研究了社会优势对显性和非显性雄性激素水平和垂体细胞数量的影响。免疫荧光研究表明，FSH-EGFP转基因鱼在FSH分泌细胞中可靠地表达EGFP。EGFP的表达模式与促黄体生成素不同。占主导地位的男性有较大的性腺，以及较高水平的雄激素和促性腺激素的血浆。显性雄性的垂体表现出较高的促性腺激素含量和基因表达。流式细胞术显示垂体增生以及FSH细胞增生和肉芽增加。总之，这些研究结果表明，促性腺激素细胞增生以及增加生产的个别细胞的基础上增加的生殖活动占主导地位的罗非鱼男性。(C)2013 Elsevier Inc. All rights reserved.
Tilapias are emerging as one of the most important fish in worldwide aquaculture and are also widely used as model fish in the study of reproduction and behavior. During the reproductive season, male tilapia are highly territorial and form spawning pits in which the dominant males court and spawn with available females. Non-territorial males stand a much lower chance of reproducing. Using transgenic tilapia in which follicle stimulating hormone (FSH) gonadotrophs were fluorescently labeled with enhanced green fluorescent protein (EGFP), we studied the effect of social dominance on the hormonal profile and pituitary cell populations in dominant and non-dominant males. Immunofluorescence studies showed that FSH-EGFP-transgenic fish reliably express EGFP in FSH-secreting cells. EGFP expression pattern differed from that of luteinizing hormone. Dominant males had larger gonads as well as higher levels of androgens and gonadotropins in the plasma. Pituitaries of dominant males exhibited higher gonadotropin content and gene expression. Flow cytometry revealed pituitary hyperplasia as well as FSH cell hyperplasia and increased granulation. Taken together, these findings suggest that gonadotroph hyperplasia as well as increased production by individual cells underlie the increased reproductive activity of dominant tilapia males. (C) 2013 Elsevier Inc. All rights reserved.