Feeding Aspergillus protease preparation combined with adequate protein diet to rats increases levels of cecum gut-protective amino acids, partially linked to Bifidobacterium and Lactobacillus
Feeding Aspergillus protease preparation combined with adequate protein diet to rats increases levels of cecum gut-protective amino acids, partially linked to Bifidobacterium and Lactobacillus
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DOI：
10.1080/09168451.2019.1627183
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发表时间：
2019-06
期刊：
影响因子：
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通讯作者：
Yongshou Yang;Thanutchaporn Kumrungsee;Manabu Kuroda;S. Yamaguchi;N. Kato
中科院分区：
文献类型：
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作者：
Yongshou Yang;Thanutchaporn Kumrungsee;Manabu Kuroda;S. Yamaguchi;N. Kato
摘要我们最近的研究表明，膳食米曲霉蛋白酶制剂（AP），通过其酶活性，在大鼠体内产生生物合成效应。我们假设，饮食AP链接到蛋白质降解，随后提高肠道保护氨基酸（AAs）在大鼠喂养足够的蛋白质饮食。在这项研究中，饮食AP显着增加双歧杆菌和乳酸杆菌的相对丰度和水平的游离苏氨酸，丙氨酸，脯氨酸，牛磺酸，鸟氨酸，苯丙氨酸，胱氨酸，和γ-氨基丁酸在盲肠内容物中喂养了足够的蛋白质饮食，但没有在那些喂养了低蛋白饮食。除鸟苷酸和苯丙氨酸外，AA升高可能对肠道相关的健康有益。一些AP调制的游离氨基酸似乎与双歧杆菌和乳酸杆菌的相对丰度有关。因此，AP与足够的蛋白质饮食相结合可能会增加盲肠有益游离AA的水平，这部分与益生菌的相对丰度相关。曲霉蛋白酶制剂与充足的蛋白质饮食一起增加了与双歧杆菌和乳酸杆菌相关的肠道保护性氨基酸的水平。
ABSTRACT Our recent study indicated that dietary Aspergillus oryzae-derived protease preparation (AP), through its enzymatic activity, exerted a bifidogenic effect in rats. We hypothesized that dietary AP links to protein degradation and subsequently elevates gut-protective amino acids (AAs) in rats fed adequate protein diet. In this study, dietary AP markedly increased the relative abundance of Bifidobacterium and Lactobacillus and the levels of free threonine, alanine, proline, taurine, ornithine, phenylalanine, cystine, and γ-aminobutyric acid in the cecum contents of rats fed with an adequate protein diet, but not in those fed with a low-protein diet. The elevated AAs, except ornithine and phenylalanine, potentially have gut-related health benefits. Some of the AP-modulated free AAs appeared to be associated with the relative abundance of Bifidobacterium and Lactobacillus. Thus, AP combined with adequate protein diet is likely to increase the levels of cecum beneficial free AAs, which is partially associated with the relative abundance of the probiotics. GRAPHICAL ABSTRACT Aspergillus protease preparation with adequate protein diet increases levels of gut protective amino acids associated with Bifidobacterium and Lactobacillus.