Matrix-free desorption of biomolecules using massive cluster impact.
Matrix-free desorption of biomolecules using massive cluster impact.
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DOI：
10.1002/rcm.1290081218
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发表时间：
1994-12
期刊：
影响因子：
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通讯作者：
D. Cornett;Terry D. Lee;J. Mahoney;P. Todd
中科院分区：
文献类型：
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作者：
D. Cornett;Terry D. Lee;J. Mahoney;P. Todd
发现大团簇冲击（MCI）电离能够在不使用液体基质的情况下从生物样品中产生高二次离子产量。观察到细胞色素c分子的干燥样品被甘油簇的MCI光束有效地解吸而没有明显的碎裂。此外，质子化分子发出的信号即使在暴露于主光束几分钟后，仍保持了相当大比例的初始强度。经确定，这些显著的现象并不是由集束轰炸造成的一层甘油堆积的结果。
Massive-cluster impact (MCI) ionization was found to be capable of producing high secondary-ion yields from biological samples without the use of a liquid matrix. Dry samples of molecules as large as cytochrome c were observed to be efficiently desorbed without significant fragmentation by the MCI beam of glycerol clusters. In addition, signals from protonated molecules retained a significant proportion of their initial intensity even after several minutes exposure to the primary beam. It was determined that these remarkable phenomena are not the result of the build-up of a layer of glycerol caused by the cluster bombardment.