Noninvasive biophotonic imaging for studies of infectious disease.
Noninvasive biophotonic imaging for studies of infectious disease.
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DOI：
10.1111/j.1574-6976.2010.00252.x
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发表时间：
2011-03
影响因子：
11.3
通讯作者：
Wiles S
中科院分区：
文献类型：
--
作者：
Andreu N;Zelmer A;Wiles S
据世界卫生组织估计，传染性生物体造成全球约四分之一的死亡。动物模型在疫苗和治疗剂的开发中发挥着重要作用，但需要大量动物通过组织采样来获得定量的微生物数据。生物光子成像 (BPI) 是一种高度灵敏、无毒的技术，基于使用敏感光子探测器检测由荧光素酶催化反应（生物发光）或荧光分子激发产生的可见光。因此，生物发光/荧光微生物的发展允许对完整活体动物内的微生物进行实时无创检测。在整个实验过程中对同一动物进行多次成像可以极其准确地跟踪疾病进展，从而减少产生具有统计意义的数据所需的动物数量。在传染病研究中，BPI 的使用变得越来越广泛，因为它可以为已建立的模型提供新颖的见解，以及该技术对在研究中使用动物的两个指导原则（即减少和细化）的影响。在这里，我们回顾了 BPI 技术，从仪器仪表到光子信号的生成，并说明了该技术如何揭示体内感染动态。
According to World Health Organization estimates, infectious organisms are responsible for approximately one in four deaths worldwide. Animal models play an essential role in the development of vaccines and therapeutic agents but large numbers of animals are required to obtain quantitative microbiological data by tissue sampling. Biophotonic imaging (BPI) is a highly sensitive, nontoxic technique based on the detection of visible light, produced by luciferase-catalysed reactions (bioluminescence) or by excitation of fluorescent molecules, using sensitive photon detectors. The development of bioluminescent/fluorescent microorganisms therefore allows the real-time noninvasive detection of microorganisms within intact living animals. Multiple imaging of the same animal throughout an experiment allows disease progression to be followed with extreme accuracy, reducing the number of animals required to yield statistically meaningful data. In the study of infectious disease, the use of BPI is becoming widespread due to the novel insights it can provide into established models, as well as the impact of the technique on two of the guiding principles of using animals in research, namely reduction and refinement. Here, we review the technology of BPI, from the instrumentation through to the generation of a photonic signal, and illustrate how the technique is shedding light on infection dynamics in vivo.
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影响因子：
3.7
作者：
Alieva NO;Konzen KA;Field SF;Meleshkevitch EA;Hunt ME;Beltran-Ramirez V;Miller DJ;Wiedenmann J;Salih A;Matz MV
通讯作者：
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通讯作者：
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通讯作者：
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通讯作者：
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作者：
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通讯作者：
Griffiths, MW