Killer cells: a functional comparison between natural, immune T-cell and antibody-dependent in vitro systems.
Killer cells: a functional comparison between natural, immune T-cell and antibody-dependent in vitro systems.
复制标题
DOI：
10.1084/jem.143.4.772
复制
发表时间：
1976-04-01
期刊：
影响因子：
--
通讯作者：
Wigzell H
中科院分区：
文献类型：
--
作者：
Kiessling R;Petranyi G;Kärre K;Jondal M;Tracey D;Wigzell H
以前的报告表明，某些品系的非免疫成年小鼠的脾细胞在短期51 Cr释放试验中确实定期杀死莫洛尼白血病病毒诱导的淋巴瘤。这种自然产生的杀伤（NK）细胞具有低粘附特性，并具有淋巴细胞的形态学外观。它仍然缺乏成熟T或B淋巴细胞的表面特征。在本报告中，进行了功能研究，平行比较NK系统、穿过H-2屏障的T细胞杀伤（抗P815）和抗体依赖性细胞介导的鸡红细胞（CRBC）系统。与CRBC系统中的效应细胞相比，NK细胞对红细胞抗体补体（EAC）花环耗竭不敏感，并且可以通过尼龙羊毛柱。NK活性不受热聚集的人或小鼠丙种球蛋白的存在下，在CRBC系统中注意到的强烈抑制相反。NK系统和免疫P815系统对胰蛋白酶预处理敏感，而CRBC系统对胰蛋白酶相对耐受。抗θ抗体加补体可消除抗P815活性，但不影响NK活性。因此，本结果进一步区分了NK细胞与细胞毒性T淋巴细胞或抗体依赖性杀伤细胞。
Previous reports have shown that spleen cells from nonimmune adult mice of certain strains do regularly kill Moloney leukemia virus-induced lymphomas in short-term 51Cr release assays. This naturally occuring killer (NK) cell had low adherent properties and had the morphological appearance of a lymphocyte. Still it lacked surface characteristics of mature T or B lymphocytes. In the present report a functional study was carried out, comparing in parallel the NK system, the T-cell killing across an H-2 barrier (anti-P815), and the antibody-dependent cell- mediated chicken red blood cell (CRBC) system. In contrast to the effector cells in the CRBC system, the NK cells were insensitive to erythrocyte antibody complement (EAC) rosette depletion and would pass through nylon wool columns. NK activity was not inhibited by the presence of heat-aggregated human or mouse gamma globulin, in contrast to the strong inhibition noted in the CRBC system. Sensitivity to trypsin pretreatment was noted in the NK system as well as in the immune P815 system, whereas the CRBC system was relatively trypsin resistant. Antitheta plus complement eliminated the anti-P815 activity, but did not touch the NK activity. The present results thus further distinguish the NK cell from cytotoxic T lymphocytes or from antibody- dependent killer cells.