LAMBDA REPRESSOR CONTAINS 2 DOMAINS
LAMBDA REPRESSOR CONTAINS 2 DOMAINS
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DOI：
10.1073/pnas.76.4.1608
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
PTASHNE, M
中科院分区：
文献类型：
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作者：
PABO, CO;SAUER, RT;PTASHNE, M
木瓜酶消化.lambda。噬菌体抑制因子产生2个相对抵抗进一步消化的片段。一个包括氨基[N]末端(残基1-92)和另一个C末端(残基132-236)。量热分析表明，N-末端片段在50℃附近变性。C-末端片段在70℃附近变性。完整的抑制子经历了2次变性，其中一次接近50度。C和另一个接近70度的温度。C.这些和其他数据表明.lambda。抑制子由两个结构域组成，由一个40个氨基酸长的连接体连接，该连接体对蛋白酶敏感。N-末端结构域与DNA结合，C-末端结构域寡聚。
Papain digestion of the .lambda. phage repressor produces 2 fragments that are relatively resistant to further digestion. One includes the amino [N] terminus (residues 1-92) and the other the C terminus (residues 132-236). Calorimetry shows that the N-terminal fragment denatures near 50.degree. C and that the C-terminal fragment denatures near 70.degree. C. Intact repressor undergoes 2 denaturations, one near 50.degree. C and another near 70.degree. C. These and other data show that .lambda. repressor consists of 2 domains joined by a connector 40 amino acids long that is sensitive to proteases. The N-terminal domain binds DNA, and the C-terminal domain oligomerizes.