The Relationships Between Toxicity, Species and Populations in Nassarius based on Toxin Detection and Multiple Gene Barcoding
The Relationships Between Toxicity, Species and Populations in Nassarius based on Toxin Detection and Multiple Gene Barcoding
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基于毒素检测和多基因条形码的织纹藻毒性、物种和种群之间的关系
DOI：
10.1007/s11802-019-3921-7
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
WANG Changhai
中科院分区：
文献类型：
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作者：
ZOU Shanmei;SONG Jiameng;WANG Chun;WANG Changhai
纳萨留斯在中国和其他亚洲国家造成了许多中毒事件。罗非鱼的毒性与种类和种群有关。然而，不同毒性的纳氏藻样的分子归属尚不清楚，这加剧了中毒情况。本研究结合毒性、毒素检测和多基因DNA条形码对中国沿海各种有毒的纳氏藻种群进行了划分。毒力试验表明，不同种内不同种群和同一种内不同种群具有不同的毒性。经高效液相色谱鉴定，该毒素为河豚毒素。不同样品的毒素含量不同。COI、16S和ITS序列显示不同毒力的Nassarius种为单系分支。所有测试的样本都已被归类为物种水平。但不同的毒力种群并没有形成独立的分支，有毒和无毒的种群也没有形成两个独立的分支。本研究基于毒性检测和多基因DNA条码技术，构建了不同毒力的纳氏藻样的毒性与分类归属的关系，为软体动物的毒力控制和抗性差异的研究提供了依据。研究还表明，水蚤的毒性在遗传水平上确实与物种有关，但与种群没有绝对的关系。
Nassarius causes many poisonings in China and other Asian countries. The toxicity of Nassarius is related to species and popu-lations. However, the molecular assignment of different toxic Nassarius samples is still unclear, which deteriorates the poisoning. Our study combined toxicity, toxin detection and multiple genes DNA barcoding to assign the various toxic Nassarius species and populations from China coast. The toxicity test indicated that different Nassarius species and different populations within one Nassarius species had different toxicity. Based on High Performance Liquid Chromatography, the toxin was confirmed to be TTX. The toxin content varied in different samples. The COI, 16S and ITS sequences revealed different toxic Nassarius species as monophyletic clades. All the samples tested have been assigned to species level. However, the different toxic populations within N. succinctus, N. variciferus and N. semiplicata did not form separated clades, and the toxic and nontoxic species did not form two clades separately. Our study constructed the relationship between toxicity and taxonomy assignment of different toxic Nassarius samples based on toxicity detection and multiple gene DNA barcoding, which provides the basis for controlling poisoning and studying the toxin-resistance difference in Mollusca. It is also indicated that the toxicity of Nassarius is really related with species at the genetic level, but not absolutely related with populations..