The contribution of ionic currents to changes in refractoriness of human atrial myocytes associated with chronic atrial fibrillation
The contribution of ionic currents to changes in refractoriness of human atrial myocytes associated with chronic atrial fibrillation
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DOI：
10.1016/s0008-6363(01)00380-7
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发表时间：
2001-11-01
影响因子：
10.8
通讯作者：
Rankin, AC
中科院分区：
文献类型：
--
作者：
Workman, AJ;Kane, AK;Rankin, AC
目的：探讨慢性心房颤动(房颤)时人心房单细胞功能电生理特性的变化及伴随离子电流变化对此的影响。方法：采用全细胞膜片钳技术记录动作电位。对11例慢性(6个月)房颤患者和39例窦性心律患者用酶法分离的心肌细胞，在无药物和有药物存在的情况下，测定有效不应期(ERP)和离子电流。结果：高频率刺激(高达600次/分)显著缩短窦性心律患者细胞的晚期复极和ERP，并使最大舒张期电位(MDP)去极化。慢性房颤与生理性事件相关电位的降低(从203+/-16到104+/-15ms，P
Objective: To investigate changes in human atrial single cell functional electrophysiological properties associated with chronic atrial fibrillation (AF), and the contribution to these of accompanying ion current changes. Methods: The whole cell patch clamp technique was used to record action potentials. the effective refractory period (ERP) and ion currents, in the absence and presence of drugs, in enzymatically isolated myocyte, from 11 patients with chronic (>6 months) AF and 39 patients in sinus rhythm. Results: Stimulation at high rates (up to 600 beats/min) markedly shortened late repolarisation and the ERP in cells from patients in sinus rhythm, and depolarised the maximum diastolic potential (MDP). Chronic AF was associated with a reduction in the ERP at physiological rate (from 203 +/- 16 to 104 +/- 15 ms, P