Lycium barbarum polysaccharide inhibits the infectivity of Newcastle disease virus to chicken embryo fibroblast
Lycium barbarum polysaccharide inhibits the infectivity of Newcastle disease virus to chicken embryo fibroblast
复制标题
枸杞多糖抑制新城疫病毒对鸡胚成纤维细胞的感染作用
DOI：
10.1016/j.ijbiomac.2009.11.011
复制
发表时间：
2010-03-01
影响因子：
8.2
通讯作者：
Guo, Liwei
中科院分区：
文献类型：
--
作者：
Wang, Junmin;Hu, Yuanliang;Guo, Liwei
采用水煎醇沉法提取枸杞多糖（LBPS），经纯化后，采用氯磺酸-吡啶法在4种改性条件下分别制备了4种硫酸化枸杞多糖（sLBPS）：sLBPS（0）（7）、sLBPS（1 1）、sLBPS（1 5）和sLBPS（1 9）。将安全浓度范围内的5个浓度的4种sLBPSs和纽卡斯尔病毒（NDV）分别以先加多糖、后加多糖和混合后同时加多糖和病毒3种方式加入到鸡胚成纤维细胞（CEF）培养体系中。以未修饰的LBPS为对照，用MTT法检测sLBPS对NDV细胞感染性的影响。结果表明，sLBPS（1.5）、sLBPS（1.9）和sLBPS（1.1）在3种加样方式下，sLBPS（0 7）混合后同时加入可显著抑制NDV对CEF的感染力，sLBPS（1）（5）预处理的病毒抑制率为10 0%，sLBPS（0 7）预处理的病毒抑制率为10%。同时添加和后添加的sLBPS（1 9）最高。未改性的LBPS在任何加样方式下均未表现出显著的效果。结果表明，LBPS经硫酸酯化改性后，抗病毒活性显著提高，且与sLBPS的硫酸酯化度（DS）有关。sLBPS（1 5）和sLBPS（1 9）具有较好的抗病毒活性，可作为抗病毒方剂的组成成分。(C)2009爱思唯尔B V版权所有
Lycium barbarum polysaccharide (LBPS) was extracted by water decoction and ethanol precipitation After purification, four sulfated lycium barbarum polysaccharides (sLBPSs), sLBPS(0) (7) , sLBPS(1 1), sLBPS(1 5) and sLBPS(1 9), were prepared by chlorosulfonic acid-pyridine method respectively at four designed modification conditions. Four sLBPSs at 5 concentrations, within the safety concentration scope, and Newcastle disease virus (NDV) were added into cultivating system of chick embryo fibroblast (CEF) respectively in three modes, pre- and post-adding polysaccharide and simultaneous adding polysaccharide and virus after being mixed. The effects of sLBPSs on cellular infectivity of NDV were assayed by MTT method taking the non-modified LBPS as control The results showed that sLBPS(1 5), sLBPS(1 9) and sLBPS(1 1) in three sample-adding modes, sLBPS(0 7) in simultaneous adding after being mixed could significantly inhibit the infectivity of NDV to CEF The viral inhibitory rate of sLBPS(1) (5) in pre- and simultaneous adding and sLBPS(1 9) in post-adding was the highest. Non-modified LBPS did not present significant effect in any sample-adding mode. These results indicated that sulfated modification could significantly enhance the antiviral activity of LBPS, which was correlated with the degree of sulfation (DS) of sLBPS sLBPS(1 5) and sLBPS(1 9) possessed better activity and would be as the compositions of antiviral prescription. (C) 2009 Elsevier B V All rights reserved