In vivo magnetic resonance imaging of transgene expression
In vivo magnetic resonance imaging of transgene expression
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DOI：
10.1038/73219
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发表时间：
2000-03-01
期刊：
影响因子：
82.9
通讯作者：
Basilion, James P.
中科院分区：
文献类型：
--
作者：
Weissleder, Ralph;Moore, Anna;Basilion, James P.
ResultsFirst, we sought to demonstrate that ETR expression correlated with increases in internalized Tf-MION. For these experiments, we implanted nude mice with both stably transfected (ETR+) and control transfected (ETR–) 9L gliosarcoma cells. Each mouse (n= 7) was implanted with both types of tumors in each flank so that it could serve as its own control. By day 12, all animals had developed tumors 200–400 mg in size. Analysis of a subset of these animals (n= 4) showed that only transfected tumors (ETR+) overexpressed the engineered transferrin receptor relative to control tumors (ETR–) derived from the same animal (Fig. 2a and b). ETR+ tumors had more iron, as measured by staining, than did ETR–tumors (Fig. 2c and d). Nuclear imaging of explanted tumors from mice treated with111In-Tf-MION also
