Hypocrellin-Loaded Gold Nanocages with High Two-Photon Efficiency for Photothermal/Photodynamic Cancer Therapy in Vitro
Hypocrellin-Loaded Gold Nanocages with High Two-Photon Efficiency for Photothermal/Photodynamic Cancer Therapy in Vitro
复制标题
具有高双光子效率的竹红菌素金纳米笼用于体外光热/光动力癌症治疗
DOI：
10.1021/nn302634m
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发表时间：
2012-09-01
期刊：
影响因子：
17.1
通讯作者：
Li, Junbai
中科院分区：
文献类型：
--
作者：
Gao, Liang;Fei, Jinbo;Li, Junbai
利用光敏剂掺入的混合脂质包裹的金纳米笼构建了一种新的生物共轭纳米结构，用于体外高效的双光子光热/光动力治疗癌症。扫描电子显微镜和透射电子显微镜的图像显示，所制备的前体和生物缀合物纳米结构是窄分散的，具有均匀的形貌。能量色散X射线分析和紫外-可见光谱表明，上述生物共轭纳米结构组装成功，并在近红外区具有较强的吸收。荧光和电子自旋共振结果表明，在生物共轭纳米结构中的金纳米笼可以显着淬灭光敏剂，并抑制单线态氧的产生，这应该是减轻光敏剂的不必要的副作用源于他们的非靶向分布。我们已经证明，由于纳米复合物被癌细胞内化，在双光子照射下，光热效应显著增强了光动力抗癌治疗。
A new bioconjugate nanostructure was constructed by using photosensitizer-incorporated mixed lipid-coated gold nanocages for two-photon photothermal/photodynamic cancer therapy in vitro with high efficiency. Scanning electron microscopic and transmission electron microscopic images reveal that the precursors and bioconjugate nanostructure as-prepared are narrowly dispersed and possess uniform morphologies. The relevant energy dispersion X-ray analysis and UV-vis spectra indicate that the bioconjugate nanostructure above was assembled successfully and has a strong absorption in the near-infrared region. Fluorescence and electronic spin resonance results show that the gold nanocage in the bioconjugate nanostructure can dramatically quench the photosensitizer and inhibit the production of singlet oxygen, which is supposed to alleviate the photosensitizers' unwanted side effects originating from their nontargeted distribution. We have demonstrated that as the nanocomplex is internalized by cancer cells, under two-photon illumination, photodynamic anticancer treatment is dramatically enhanced by the photothermal effect.