Histone deacetylase inhibitor treated cell sheet from mouse tendon stem/progenitor cells promotes tendon repair
Histone deacetylase inhibitor treated cell sheet from mouse tendon stem/progenitor cells promotes tendon repair
复制标题
组蛋白脱乙酰酶抑制剂处理的小鼠肌腱干/祖细胞细胞片促进肌腱修复
DOI：
10.1016/j.biomaterials.2018.03.043
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发表时间：
2018
期刊：
影响因子：
14
通讯作者：
Ouyang Hongwei
中科院分区：
文献类型：
--
作者：
Zhang Can;Zhang Erchen;Yang Long;Tu Wenjing;Lin Junxin;Yuan Chunhui;Bunpetch Varisara;Chen Xiao;Ouyang Hongwei
Tendon stem/progenitor cells (TSPCs) have been identified as a rare population in tendons.In vitropropagation is indispensable to obtain sufficient quantities of TSPCs for therapies. However, culture-expanded TSPCs are prone to lose their phenotype, resulting in an inferior repaired capability. And little is known about the underlying mechanism. Here, we found that altered gene expression was associated with increased histone deacetylase (HDAC) activity and expression of HDAC subtypes. Therefore, we exposedScxGFPmice-derived TSPCs to HDAC inhibitor (HDACi) trichostatin A (TSA) or valproic acid (VPA), and observed significant expansion ofScxGFP+cells without altering phenotypic properties. TSA upregulatedScxexpression by inhibiting HDAC1 and -3, and increasing the H3K27Ac level ofTgfb1and -2genome region. Additionally, cell sheets formed from TSA-pretreated mTSPCs retained the ability to accelerate tendon repairin vivo. Thus, our results uncovered an unrecognized role of HDACi in phenotypic and functional mTSPCs expansion to enhance their therapeutic potential.
