Facile preparation of a fluorescent probe to detect the cellular ability of nucleotide excision repair.
Facile preparation of a fluorescent probe to detect the cellular ability of nucleotide excision repair.
复制标题
轻松制备荧光探针来检测细胞核苷酸切除修复的能力。
DOI：
10.1016/j.ab.2017.03.023
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Iwai S.
中科院分区：
文献类型：
--
作者：
Tawarahara H;Kuraoka I;Iwai S.
我们以前开发了一种方法来检测细胞的核苷酸切除修复的能力，其功能是去除紫外线诱导的DNA损伤，使用质粒型荧光探针。这种方法普遍使用的一个缺点是，在质粒制备中用作引物的含有（6-4）光产物的寡核苷酸必须化学合成。在这项研究中，我们使用合成后的紫外线照射的寡核苷酸作为引物制备探针。细胞的转染证明该探针以与原始探针相同的方式检测细胞的修复能力。
We previously developed a method to detect the cellular ability of nucleotide excision repair, which functions to remove UV-induced lesions in DNA, using a plasmid-type fluorescent probe. A drawback to the popular use of this method was that the oligonucleotide containing the (6–4) photoproduct, which was used as a primer in the plasmid preparation, must be synthesized chemically. In this study, we prepared the probe using a post-synthetically UV-irradiated oligonucleotide as the primer. Transfection of cells demonstrated that this probe detected the repair ability of the cells in the same manner as the original probe.