CHFR is important for the survival of male premeiotic germ cells.
CHFR is important for the survival of male premeiotic germ cells.
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CHFR 对于雄性减数分裂前生殖细胞的存活很重要。
DOI：
10.1080/15384101.2015.1093701
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发表时间：
2015
期刊：
影响因子：
4.3
通讯作者：
Yu Xiaochun
中科院分区：
文献类型：
--
作者：
Lu Lin-Yu;Yu Xiaochun
DNA损伤反应是男性生育能力所必需的。DNA损伤修复介导减数分裂前期同源染色体间的重组，是减数分裂过程中染色体分离的关键。有趣的是，一些DNA损伤反应蛋白也是减数前生殖细胞存活所必需的，但它们在这些细胞中的作用尚不清楚。最近有研究表明CHFR参与DNA损伤反应，但男性生育是否需要CHFR仍有待确定。在这项研究中，我们对基因敲除的雄性小鼠进行了表征，发现其中约30%的小鼠不育。精子发生延迟，减数分裂前生殖细胞凋亡显著增加。这导致这些小鼠在3个月内睾丸中生殖细胞的完全丧失和无精子症。我们进一步证明，ATM的激活在这些小鼠的睾丸中受到损害。因此，CHFR对男性早衰生殖细胞的存活很重要，这可能是通过维持精原干细胞的基因组稳定性来实现的。
DNA damage response is required for male fertility. DNA damage repair mediates recombination between homologous chromosomes in meiotic prophase, which is essential for proper chromosome segregation during meiotic division. Interestingly, some DNA damage response proteins are also required for the survival of premeiotic germ cells, but their roles in these cells are still unclear. CHFR was recently shown to participate in DNA damage response, but it remains to be established if CHFR is required for male fertility. In this study, we characterizedChfrknockout male mice and found that around 30% of them were infertile. The onset of spermatogenesis was delayed and there was significant increase in apoptosis in premeiotic germ cells. This resulted in complete loss of germ cells in testes in 3 months and azoospermia in these mice. We further demonstrated that ATM activation was compromised in the testes of these mice. Therefore, CHFR is important for the survival of male premeiotic germ cells, which is likely through maintaining genomic stability in spermatogonial stem cells.