Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples.
Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples.
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DOI：
10.2116/analsci.32.35
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Lunte SM
中科院分区：
文献类型：
--
作者：
Oborny NJ;Costa EE;Suntornsuk L;Abreu FC;Lunte SM
开发了一种用于微芯片电泳的便携式荧光检测系统，并与台式系统进行了比较。利用该系统，将脑透析液中常见的6种神经活性胺——精氨酸、瓜氨酸、牛磺酸、组胺、谷氨酸和天冬氨酸——与萘-2,3-二甲醛/氰化物脱机衍生，电泳分离，荧光检测。台式系统的检出限为50nM ~ 250nM，便携式系统的检出限为250nM ~ 1.3 μM，适用于脑微透析样品的分析物检测。然后演示了便携式系统对大鼠脑微透析样品中相同的六种胺的检测。
A portable fluorescence detection system for use with microchip electrophoresis was developed and compared to a benchtop system. Using this system, six neuroactive amines commonly found in brain dialysate—arginine, citrulline, taurine, histamine, glutamate, and aspartate—were derivatized offline with naphthalene-2,3-dicarboxaldehyde/cyanide, separated electrophoretically, and detected by fluorescence. Limits of detection for the analytes of interest were 50nM – 250nM for the benchtop system and 250 nM – 1.3 μM for the portable system, both of which were adequate for analyte determination in brain microdialysis samples. The portable system was then demonstrated for the detection of the same six amines in a rat brain microdialysis sample.