IDENTIFICATION AND CHARACTERIZATION OF MPG1, A GENE INVOLVED IN PATHOGENICITY FROM THE RICE BLAST FUNGUS MAGNAPORTHE-GRISEA
IDENTIFICATION AND CHARACTERIZATION OF MPG1, A GENE INVOLVED IN PATHOGENICITY FROM THE RICE BLAST FUNGUS MAGNAPORTHE-GRISEA
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DOI：
10.1105/tpc.5.11.1575
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发表时间：
1993-11-01
期刊：
影响因子：
11.6
通讯作者：
HAMER, JE
中科院分区：
文献类型：
--
作者：
TALBOT, NJ;EBBOLE, DJ;HAMER, JE
利用差异cDNA克隆技术鉴定了稻瘟病菌在其寄主水稻中侵染生长过程中表达的基因。我们详细描述了其中一个基因MPG1。使用一种新的测定方法来确定植物中存在的真菌生物量的比例，我们确定MPG 1转录物在植物生长期间的丰度是在培养物中的60倍。Mpg1突变体引起疾病症状的能力降低，这似乎是由于附着胞形成能力受损所致。MPG1 mRNA是非常丰富的，伴随着附着胞形成的植物感染的早期，也是丰富的症状发展的时候。MPG1 mRNA也表达在分生孢子和菌丝培养物饥饿的氮或碳。MPG1可能编码一种小的、分泌的、富含半胱氨酸的、具有真菌疏水蛋白特征的适度疏水蛋白。与MPG1基因产物作为疏水蛋白的作用一致，Mpg1突变体显示出“易润湿”表型。我们的研究结果表明，疏水蛋白可能有一个作用，在真菌的感染结构的阐述，并可能履行其他功能，真菌植物病变。
Differential cDNA cloning was used to identify genes expressed during infectious growth of the fungal pathogen Magnaporthe grisea in its host, the rice plant. We characterized one of these genes, MPG1, in detail. Using a novel assay to determine the proportion of fungal biomass present in the plant, we determined that the MPG1 transcript was 60-fold more abundant during growth in the plant than in culture. Mpg1 mutants have a reduced ability to cause disease symptoms that appears to result from an impaired ability to undergo appressorium formation. MPG1 mRNA was highly abundant very early in plant infection concomitant with appressorium formation and was also abundant at the time of symptom development. The MPG1 mRNA was also expressed during conidiation and in mycelial cultures starved for nitrogen or carbon. MPG1 potentially encodes a small, secreted, cysteine-rich, moderately hydrophobic protein with the characteristics of a fungal hydrophobin. Consistent with the role of the MPG1 gene product as a hydrophobin, Mpg1 mutants show an ''easily wettable'' phenotype. Our results suggest that hydrophobins may have a role in the elaboration of infective structures by fungi and may fulfill other functions in fungal phytopathogenesis.