Sequence requirements for Afr-2 regulation of alpha-fetoprotein gene expression during liver regeneration.
Sequence requirements for Afr-2 regulation of alpha-fetoprotein gene expression during liver regeneration.
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肝再生过程中 Afr-2 调节甲胎蛋白基因表达的序列要求。
DOI：
10.1007/bf02369911
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Feuerman,MH
中科院分区：
文献类型：
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作者：
Jin,DK;Feuerman,MH
甲胎蛋白（AFP）基因表达发生在卵黄囊，胎儿肝脏和肠道，并在成人肝脏再生和肿瘤发生。近交系小鼠品系C3 H/He和C57 Bl/6之间在单个遗传位点Afr-2（以前称为Rif）的多态性导致肝再生期间AFP表达的不同水平。我们研究了AFP，组蛋白H3和白蛋白基因表达在肝再生过程中，发现AFP基因表达的菌株特异性差异不能归因于分裂细胞数量的差异。转基因小鼠的实验表明，Afr-2调控所需的序列包括172 bp之间的-1010和-838 bp和118 bp的AFP转录起始位点的上游，在肝再生过程中诱导所需的相同区域。这表明Afr-2表型可能源于肝再生过程中调节基因表达的基因中的等位基因差异。
Alpha-fetoprotein (AFP) gene expression occurs in the yolk sac, fetal liver and gut, and in the adult liver during regeneration and tumorigenesis. Polymorphism at a single genetic locus, Afr-2 (formerly known as Rif) between inbred mouse strains C3H/He and C57Bl/6, results in different levels of AFP expression during liver regeneration. We examined AFP, histone H3, and albumin gene expression during liver regeneration and found that the strain-specific variance in AFP gene expression could not be attributed to a difference in the numbers of dividing cells. Experiments with transgenic mice revealed sequences required for Afr-2 regulation included 172 bp between — 1010 and — 838 bp and 118 bp immediately upstream of the AFP transcriptional start site—the same regions required for induction during liver regeneration. This suggests that the Afr-2 phenotype may stem from an allelic difference in a gene regulating gene expression during liver regeneration.