Detection of amino acids as possible promoters of bladder cancer in rats by measuring their enhancement of agglutination of bladder cells by concanavalin A.
Detection of amino acids as possible promoters of bladder cancer in rats by measuring their enhancement of agglutination of bladder cells by concanavalin A.
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通过测量伴刀豆球蛋白 A 对膀胱细胞凝集的增强作用来检测氨基酸作为大鼠膀胱癌的可能促进剂。
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
T. Sugimura
中科院分区：
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作者：
T. Kakizoe;H. Komatsu;Y. Honma;T. Niijima;T. Sugimura
用N-丁基-N-（4-羟丁基）亚硝胺亚致癌处理诱导伴刀豆球蛋白A增强膀胱细胞的凝集性，研究了氨基酸对这种凝集性增强的影响。检查的氨基酸为L-丙氨酸、L-精氨酸、L-天冬酰胺、L-天冬氨酸、L-半胱氨酸、L-谷氨酸、L-谷氨酰胺、L-甘氨酸、DL-和L-组氨酸、L-羟脯氨酸、L-异亮氨酸、D-和L-亮氨酸、L-赖氨酸、L-甲硫氨酸、DL-和L-苯丙氨酸、L-脯氨酸、L-丝氨酸、L-苏氨酸、DL-、D-和L-色氨酸、L-酪氨酸以及D-和L-缬氨酸。将它们以2.0%的浓度添加到粉末状饲料中。L-亮氨酸、L-异亮氨酸、L-缬氨酸、DL-色氨酸和D-色氨酸延长了膀胱细胞与伴刀豆球蛋白A的凝集性增强的时间。蛋白酶抑制剂亮抑酶肽和L-亮氨酰-L-亮氨酸也在0.1%的浓度下进行了检查，因为它们具有相似的化学结构，并且发现具有相同的效果。已经通过体内致癌实验确定了DL-色氨酸和亮抑酶肽的促肿瘤作用。通过该短期测定检测的L-亮氨酸、L-异亮氨酸、L-缬氨酸、D-色氨酸和L-亮氨酰-L-亮氨酸的作用表明，这些化合物也可能是大鼠膀胱癌的促进剂。
The effects of amino acids on the enhanced agglutinability of bladder cells with concanavalin A induced by subcarcinogenic treatment with N-butyl-N-(4-hydroxybutyl)nitrosamine were examined. The amino acids examined were L-alanine, L-arginine, L-asparagine, L-aspartic acid, L-cysteine, L-glutamic acid, L-glutamine, L-glycine, DL- and L-histidine, L-hydroxyproline, L-isoleucine, D- and L-leucine, L-lysine, L-methionine, DL- and L-phenylalanine, L-proline, L-serine, L-threonine, DL-, D- and L-tryptophan, L-tyrosine and D- and L-valine. They were added to powdered diet at a concentration of 2.0%. L-Leucine, L-isoleucine, L-valine, DL- and D-tryptophan prolonged the period during which the bladder cells showed enhanced agglutinability with concanavalin A. Leupeptin, a protease inhibitor, and L-leucyl-L-leucine were also examined at a concentration of 0.1% because of their similar chemical structures, and were found to have the same effect. The tumor-promoting effects of DL-tryptophan and leupeptin have already been established by in vivo carcinogenesis experiments. The effects of L-leucine, L-isoleucine, L-valine, D-tryptophan and L-leucyl-L-leucine, detected by this short term assay, suggest that these compounds may also be promoters of bladder cancer in rats.