Langerhans cells from human oral epithelium are more effective at stimulating allogeneic T cells in vitro than Langerhans cells from skin
Langerhans cells from human oral epithelium are more effective at stimulating allogeneic T cells in vitro than Langerhans cells from skin
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DOI：
10.1111/j.1365-2249.2004.02469.x
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发表时间：
2004-06-01
影响因子：
4.6
通讯作者：
Dahlgren, UI
中科院分区：
文献类型：
--
作者：
Hasséus, B;Jontell, M;Dahlgren, UI
本报告的重点是朗格汉斯细胞（LC）在皮肤和口腔粘膜上皮，这两个满足不同的抗原挑战的功能能力。比较了来自人口腔和皮肤上皮的LC在体外向T细胞提供共刺激信号的能力。口腔上皮细胞粗悬液中的LC与皮肤上皮细胞相比具有显著增强的T细胞共刺激能力。这适用于伴刀豆球蛋白A（con-A）刺激的同基因T细胞的培养物和涉及同种异体T细胞的混合上皮细胞淋巴细胞反应。如果将针对HLA-DR、-DP和-DQ的单克隆抗体添加到具有同种异体T细胞的培养物中，则口腔和皮肤上皮细胞的共刺激能力降低>70%，表明表达HLA II类的LC的参与。免疫组化显示，6%的上皮细胞是CD 1a + LC在切片从口腔和皮肤上皮。口腔上皮细胞和con-A刺激的T细胞培养物中白细胞介素（IL）-8的产生高于相应的皮肤上皮细胞作为辅助细胞的培养物。结果表明，人口腔上皮中的LC比皮肤中的LC更有效地刺激T细胞。
This report is focused on the functional capacity of Langerhans cells (LC) in the epithelium of skin and oral mucosa, which both meet different antigenic challenges. The capacity of LC from human oral and skin epithelium to provide co-stimulatory signals to T cells in vitro was compared. LC in a crude suspension of oral epithelial cells had a significantly enhanced T cell co-stimulatory capacity compared to skin epithelial cells. This applied both to cultures with concanavalin A (con-A)-stimulated syngeneic T cells and to a mixed epithelial cell lymphocyte reaction involving allogeneic T cells. The co-stimulatory capacity of oral and skin epithelial cells was reduced by >70% if monoclonal antibodies against HLA-DR, -DP and -DQ were added to the cultures with allogeneic T cells, indicating the involvement of HLA class II expressing LC. Immunohistochemistry revealed that 6% of the epithelial cells were CD1a + LC in sections from both oral and skin epithelium. Interleukin (IL)-8 production was higher in cultures of oral epithelial cells and con-A stimulated T cells than in corresponding cultures with skin epithelial cells as accessory cells. The results suggest that LC in human oral epithelium are more efficient at stimulating T cells than those of skin.