Dysbiosis of gut fungal microbiota is associated with mucosal inflammation in Crohn's disease.
Dysbiosis of gut fungal microbiota is associated with mucosal inflammation in Crohn's disease.
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DOI：
10.1097/mcg.0000000000000035
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发表时间：
2014-07
影响因子：
2.9
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Li Q;Wang C;Tang C;He Q;Li N;Li J
补充数字内容可在正文中找到。我们的目标是描述克罗恩病(CD)患者肠道粘膜和粪便中的真菌微生物区系。真菌代表了人体肠道中的多种微生物群落，可能在CD的发病机制中发挥了作用；然而，对于CD患者肠道黏膜上真菌菌群的结构和组成，尤其是附着在肠道粘膜上的真菌微生物区系，目前还知之甚少。19例活动期CD患者和7例健康体检者作为研究对象。采用独立于培养的群落指纹技术分析CD患者的黏膜相关微生物和粪便真菌。炎症黏膜中真菌的丰富度和多样性明显高于非炎症黏膜。炎症粘膜的主要真菌组成发生了显著变化，主要表现为念珠菌、串珠赤霉菌、油菜链格孢菌和新生隐球菌的比例增大。CD患者的粪便真菌群落受到干扰，伴随着白色念珠菌、棒曲霉和新生葡萄球菌中真菌多样性和流行率的增加。黏膜真菌群落的物种丰富度和多样性与肿瘤坏死因子-α、干扰素-γ和IL-10的表达有关(P<；0.05)。粪便真菌菌群多样性与血清C-反应蛋白、CD活性指数呈正相关(P<；0.05)。这项研究首先证明了炎症粘膜中的真菌微生物区系与非炎症区域的微生物区系是可区分的。肠道真菌菌群组成的改变可能与CD的黏膜炎症和疾病活动性有关。我们的数据将为理解肠道真菌在CD发病机制中的潜力提供新的见解。
Supplemental Digital Content is available in the text. We aim to characterize the fungal microbiota in the intestinal mucosa and feces in patients with Crohn’s disease (CD). Fungi represent a diverse microbial community in the human intestine and might play a role in the pathogenesis of CD; however, little is known about the structure and composition of the fungal microbiota especially adhering to the intestinal mucosa in CD patient. Nineteen patients with active CD and 7 healthy individuals were recruited in this study. The mucosa-associated and fecal fungal microbiotas in CD patients were analyzed using culture-independent community fingerprint techniques. The fungal richness and diversity were significantly elevated in the inflamed mucosa compared with the noninflamed mucosa. The predominant fungal composition in the inflamed mucosa was strikingly altered, mainly characterized by expansion in the proportions of Candida spp., Gibberella moniliformis, Alternaria brassicicola, and Cryptococcus neoformans. The fecal fungal community was perturbed in CD patients as accompanied by increased fungal diversity and prevalence in Candida albicans, Aspergillus clavatus, and C. neoformans. The species richness and diversity of the mucosal fungal community were associated with the expression of TNF-α, IFN-γ, or IL-10 (P<0.05). The diversity of the fecal fungal microbiota positively correlated with serum C-reactive protein and CD activity index (P<0.05). This study first demonstrates that the fungal microbiota in the inflamed mucosa is distinguishable from that of the noninflamed area. Shifts of gut fungal microbiota composition may be associated with mucosal inflammation and disease activity of CD. Our data would provide novel insights into understanding the potential of gut fungal microbiota in the pathogenesis of CD.