Encapsulation of proteins in hydrogel carrier systems for controlled drug delivery: Influence of network structure and drug size on release rate
Encapsulation of proteins in hydrogel carrier systems for controlled drug delivery: Influence of network structure and drug size on release rate
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DOI：
10.1016/j.jbiotec.2012.06.036
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发表时间：
2013-01-20
影响因子：
4.1
通讯作者：
Menzel, Henning
中科院分区：
文献类型：
--
作者：
Bertz, Andreas;Woehl-Bruhn, Stefanie;Menzel, Henning
以聚乙二醇甲基丙烯酸酯改性的羟乙基淀粉（HES-P（EG）（6）MA）为基质，制备了一种新型的蛋白质控释水凝胶。由于药物释放行为被认为与水凝胶网络的孔结构有关，因此通过低温-SEM测定孔径，低温-SEM是一种用于在纳米尺度上成像的温和技术。结果表明，随着聚合物浓度的增加，孔径减小，孔均匀性增加。此外，基于溶胀数据计算水凝胶的筛目尺寸。孔和网孔尺寸显著不同，这表明两种结构都存在于水凝胶中。所得结构模型与装载有FITC-葡聚糖的散装水凝胶圆柱体和装载有不同分子大小的FITC-IgG和FITC-葡聚糖的水凝胶微球的释放数据相关。初始释放在很大程度上取决于流体动力学直径与孔径之间的关系，而掺入物质的长期释放主要受小得多的网孔网络的降解控制。(C)2012爱思唯尔有限公司版权所有。
Novel hydrogels based on hydroxyethyl starch modified with polyethylene glycol methacrylate (HES-P(EG)(6)MA) were developed as delivery system for the controlled release of proteins. Since the drug release behavior is supposed to be related to the pore structure of the hydrogel network the pore sizes were determined by cryo-SEM, which is a mild technique for imaging on a nanometer scale. The results showed a decreasing pore size and an increase in pore homogeneity with increasing polymer concentration. Furthermore, the mesh sizes of the hydrogels were calculated based on swelling data. Pore and mesh size were significantly different which indicates that both structures are present in the hydrogel. The resulting structural model was correlated with release data for bulk hydrogel cylinders loaded with FITC-dextran and hydrogel microspheres loaded with FITC-IgG and FITC-dextran of different molecular size. The initial release depended much on the relation between hydrodynamic diameter and pore size while the long term release of the incorporated substances was predominantly controlled by degradation of the network of the much smaller meshes. (C) 2012 Elsevier B.V. All rights reserved.