Molecular cloning of the human angiotensin II type 2 receptor cDNA.
Molecular cloning of the human angiotensin II type 2 receptor cDNA.
复制标题
人血管紧张素 II 2 型受体 cDNA 的分子克隆。
DOI：
10.1006/bbrc.1994.2714
复制
发表时间：
1994
影响因子：
3.1
通讯作者：
Elton,TS
中科院分区：
文献类型：
--
作者：
Martin,MM;Su,B;Elton,TS
筛选成人肺cDNA文库，分离并鉴定了一个全长人血管紧张素II 2型受体（hAT 2 R）克隆。hAT 2 R cDNA克隆含有一个1089碱基对的开放阅读框架，编码363个氨基酸残基的蛋白质。hAT 2 R在序列上与大鼠和小鼠AT 2 R序列约92%相同。[125 I] CGP 42112 A的特异性结合在用hAT 2 R cDNA瞬时转染的COS-7细胞的膜中得到证实。Scatchard分析和配体置换曲线是AT 2 R的典型特征。北方分析表明，hAT 2 R mRNA在人成人肺和人胎肾中大量表达。此外，hAT 2 R mRNA仅在成人心脏和主动脉中可检测到。相反，大鼠AT 2 R mRNA在大鼠脑中大量表达，而在大鼠肺中仅可检测到。
A human adult lung cDNA library was screened and one full-length human angiotensin II type 2 receptor (hAT2R) clone was isolated and characterized. The hAT2R cDNA clone contains a 1089-base-pair open reading frame which encodes a protein of 363 amino acid residues. The hAT2R is approximately 92% identical in sequence to the rat and mouse AT2R sequences. Specific binding of [125I]CGP42112A was demonstrated in membranes from COS-7 cells transiently transfected with the hAT2R cDNA. Scatchard analysis and ligand displacement profiles were typical of the AT2R. Northern analysis demonstrated that the hAT2R mRNA was abundantly expressed in human adult lung and in human fetal kidney. Additionally, the hAT2R mRNA was just detectable in human adult heart and aorta. In contrast, the rat AT2R mRNA was abundantly expressed in the rat brain and just detectable in the rat lung.