High-Vigor Maize Seeds Resist Fusarium graminearum Infection through Stronger Ca2+ Signaling
High-Vigor Maize Seeds Resist Fusarium graminearum Infection through Stronger Ca2+ Signaling
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DOI：
10.3390/agriculture12070992
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发表时间：
2022-07
期刊：
影响因子：
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通讯作者：
Baokuan Xu;Xiyan Liu;Xuejiao Song;Q. Guo;Yongqi Yin;Chunqing Zhang;Yan Li
中科院分区：
文献类型：
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作者：
Baokuan Xu;Xiyan Liu;Xuejiao Song;Q. Guo;Yongqi Yin;Chunqing Zhang;Yan Li
高活力的种子对各种不利的环境条件有很强的抵抗力。然而，很少有人知道种子活力如何影响种子对生物胁迫的抗性。在这项研究中，新收获的具有高活力的种子和低活力的种子，通过人工加速老化处理，在接种禾谷镰刀菌24 h后用于发芽试验。结果表明，F.而与低活力种子相关的那些则被显著抑制。对表达发光Ca 2+探针的转基因玉米种子的分析表明，高活力种子胚细胞质和核中游离Ca 2+浓度显著高于低活力种子。通过Ca ~（2+）抑制剂处理和外源Ca ~（2+）处理，进一步证实了Ca ~（2+）对种子萌发和幼苗生长的重要作用。有趣的是，在F.禾谷镰刀菌（F. graminearum）种子萌发和幼苗生长所需的Ca ~（2+）主要来源于液泡钙库，而F.禾谷早熟禾所需的Ca ~（2+）主要来源于质外体钙库。本研究有助于了解高活力种子的抗病机理，为高活力种子在农业生产中的广泛应用提供理论支持。
Seeds with high vigor have strong resistance to various adverse environmental conditions. However, little is known about how seed vigor affects the resistance of seeds to biotic stress. In this study, newly harvested seeds that had high vigor and seeds with low vigor, achieved via an artificially accelerated aging treatment, were used in the germination test after inoculation with Fusariumgraminearum for 24 h. The results showed that high-vigor seed-related germination and seedling growth were not significantly affected by F. graminearum infection, while those related to low-vigor seeds were significantly inhibited. Analysis of transgenic maize seeds expressing the luminescent Ca2+ probe encoded by aequorin indicated that the concentration of free Ca2+ in the cytoplasm and nucleus of the embryo cells of high-vigor seeds was significantly higher than that of the low-vigor seeds. Through an experiment with Ca2+ inhibitor treatment and exogenous Ca2+ application, we further confirmed that Ca2+ played an important role in seed germination and seedling growth. Interestingly, in the presence of F. graminearum, the Ca2+ required for seed germination and seedling growth mainly came from the vacuolar calcium pool, while in the absence of F. graminearum, the required Ca2+ mainly came from the apoplastic calcium store. This study helps understand how high-vigor seeds resist disease and provides theoretical support for the wide application of high-vigor seeds in agricultural production.