Fractalkine expression in human renal inflammation
Fractalkine expression in human renal inflammation
复制标题
DOI：
10.1002/path.1010
复制
发表时间：
2002-01-01
影响因子：
7.3
通讯作者：
Savage, COS
中科院分区：
文献类型：
--
作者：
Cockwell, P;Chakravorty, SJ;Savage, COS
免疫性和炎症性人类肾脏疾病与大量单核细胞浸润相关。这些细胞向血管外部位的运输是由局部产生的趋化因子指导的。Fractalkine是第一个描述的细胞表面锚定趋化因子，具有有效的单核细胞定向粘附和趋化特性。本研究的目的是分析fractalkine在人类肾脏炎症中的表达和分布。原位杂交和免疫组织化学方法被用来研究肾活检15例主要肾小球炎症（血管炎性肾小球肾炎）和15例主要肾小管和间质炎症（急性肾移植排斥反应）。对照组为非炎性肾小球肾炎。和正常组织。Fractalkine mRNA主要表达于受疾病影响的肾小球或肾小管间质的主要隔室中，并且最强表达定位于炎症局部的血管部位。在急性排斥反应中，肾小管上皮细胞表达fractalkine mRNA增加。在浸润的白细胞中没有fractalkine的表达，而在对照组织中仅有稀疏的表达。Fractalkine mRNA表达与浸润性白细胞亚群相关。免疫组织化学证实了这种表达模式，连续切片共定位显示在巨噬细胞（CD68+）和T细胞（CD3+）浸润的区域中有fractalkine表达。这些表达模式表明，fractalkine是一个强有力的候选人，指导单核细胞浸润在人类肾脏炎症。版权所有（C）2001约翰威利父子有限公司
Immune and inflammatory human renal disease is associated with heavy mononuclear cell infiltration. The trafficking of these cells to extravascular sites is directed by local production of chemokines. Fractalkine is the first described cell-surface anchored chemokine and has potent mononuclear cell-directed adhesion and chemotactic properties. The purpose of this study was to analyse the expression and distribution of fractalkine in human renal inflammation. In situ hybridization and immunohistochemistry were used to study renal biopsies from 15 patients with predominant glomerular inflammation (vasculitic glomerulonephritis) and 15 with predominant tubular and interstitial inflammation (acute renal allograft rejection). Controls comprised noninflammatory glomerulonephritis. and normal tissue. Fractalkine mRNA was predominantly expressed in the major compartment, glomerular or tubulointerstitial, affected by disease and with the strongest expression localized to vascular sites local to inflammation. In acute renal allograft rejection, there was increased expression of fractalkine mRNA by tubular epithelia] cells. There was no expression of fractalkine by infiltrating leukocytes and there was only sparse expression in control tissue. Fractalkine mRNA expression correlated with infiltrating leukocyte subsets. Immunohistochemistry confirmed this pattern of expression, with serial section co-localization showing fractalkine expression in areas with macrophage (CD68+) and T cell (CD3+) infiltrates. These expression patterns show that fractalkine is a strong candidate for directing mononuclear cell infiltration in human renal inflammation. Copyright (C) 2001 John Wiley Sons, Ltd.