The type species of Amphorellopsis and Tintinnopsis (Protozoa: Ciliophora): A new ciliary pattern and some comments in Tintinnina
The type species of Amphorellopsis and Tintinnopsis (Protozoa: Ciliophora): A new ciliary pattern and some comments in Tintinnina
复制标题
Amphorellopsis 和 Tintinnopsis 的模式种（原生动物：Ciliophora）：一种新的纤毛模式和 Tintinnina 中的一些评论
DOI：
10.1016/j.jksus.2020.10.006
复制
发表时间：
2020-12-01
影响因子：
3.8
通讯作者：
Hu, Xiaozhong
中科院分区：
文献类型：
--
作者：
Bai, Yang;Wang, Rui;Hu, Xiaozhong
背景：纤毛虫作为浮游微真核生物的主要组成部分，在水生生态系统中是连接微生物食物链和传统浮游食物链的营养纽带。然而，传统上，丁宁的分类和系统学一直是基于洛里卡。现在人们承认，仅有Lorica的特征是不够的，还需要细胞学和分子信息。由于缺乏纤毛和分子信息，两属的系统分类尚不明确。结果：本研究中，两种标本均采自青岛市中国近海海域。在活体标本和蛋白染色标本观察的基础上，研究了它们的形态，并分析了它们基于SSU rDNA和LSU rDNA的系统发育。黄毛委陵菜属典型纤毛。结果表明，尖叶两性藻的体细胞运动分布均匀，没有分化为不同的纤毛区。系统发育分析表明，Tintinnopsis为非单系种，Tintinnopsis与T.nana和T.Baltica有较近的亲缘关系。结论：本研究首次揭示了两种丁宁类型物种的纤毛格局和两个核rRNA基因序列，从而扩展了丁宁的知识和数据库。两性鞭毛虫是介于胶状毛(带状)和珠状编织毛虫之间的中间血统。(C)2020年提交人(S)。由Elsevier B.V.代表沙特国王大学出版。这是CC BY-NC-ND License(http://creativecommons.org/licenses/by-nc-nd/4.0/).下的一篇开放获取文章
Background: As dominant components of planktonic microeukaryotes, the tintinnine ciliates act as a trophic link between microbial food web and traditional planktonic food chain in aquatic ecosystems. However, traditionally the taxonomy and systematics of tintinnines have been based on the lorica. It is now accepted that lorica features alone are insufficient and that cytological and molecular information are also needed. The systematics of the genera Tintinnopsis and Amphorellopsis is ambiguous, mainly due to the lack of ciliary and molecular information on their type species, T. beroidea and A. acuta.Result: In the present study, specimens of both species were collected from coastal waters of Qingdao, China. The morphology of each was investigated based on observations of live and protargol-stained specimens, and their SSU rDNAand LSU rDNA-based phylogenies were analyzed. Tintinnopsis beroidea presents the genus-typical ciliature. The somatic kineties in Amphorellopsis acuta were found to be evenly distributed without differentiation into distinct ciliary fields. The phylogenetic analyses revealed that Tintinnopsis is non-monophyletic, and that T. beroidea has a close relationship with T. nana and T. baltica. The monophyly of Amphorellopsis is also not supported by SSU rDNA analysis.Conclusion: This study first reveals the ciliary pattern and two nuclear rRNA gene sequences of two tintinnine type species, and thus expands knowledge and database of tintinnines. Amphorellopsis represents an intermediate lineage between tintinnines (loricate form) and aloricate choreotrichids. (c) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).