Estimating diversity of Indo-Pacific coral reef stomatopods through DNA barcoding of stomatopod larvae
Estimating diversity of Indo-Pacific coral reef stomatopods through DNA barcoding of stomatopod larvae
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DOI：
10.1098/rspb.2006.3540
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发表时间：
2006-08-22
影响因子：
4.7
通讯作者：
Boyce, Sarah L.
中科院分区：
文献类型：
--
作者：
Barber, Paul;Boyce, Sarah L.
人们正在努力在 25 年内全面记录世界的生物多样性。然而，这一挑战的严重程度，特别是在海洋环境中，尚不清楚。在这项研究中，我们应用 DNA 条形码来探索珊瑚三角区和红海的性蹄类口足动物（螳螂虾）的生物多样性。对 189 个未知口足类幼虫与 327 个已知成虫（代表性腺总科 67 个分类群）的序列进行比较，揭示了 22 个不同的幼虫操作分类单位 (OTU)。在西太平洋，10个幼虫OTU是性腺科和原鱿鱼科的成员，预计阳性鉴定成功率为96.5%。然而，只有五个 OTU 可以被识别为物种，并且至少三个 OTU 代表了成体形式未知的新物种。在红海，淋菌科的识别率预计为 75%，但 4 个幼虫 OTU 均无法鉴定为物种；至少有两种代表了成年形态未知的新物种。结果表明，珊瑚三角区和红海这个经过充分研究的群体的生物多样性可能被低估了至少 50% 到 150% 以上，这表明研究较少的群体面临着更大的挑战。尽管DNA条形码方法很有效，但由于新发现的参考序列数据库的分类学局限性，其总体成功受到限制，这凸显了分子遗传学家和分类学家之间的协同作用在理解和记录世界海洋和陆地环境生物多样性方面的重要性。
There is a push to fully document the biodiversity of the world within 25 years. However, the magnitude of this challenge, particularly in marine environments, is not well known. In this study, we apply DNA barcoding to explore the biodiversity of gonodactylid stomatopods (mantis shrimp) in both the Coral Triangle and the Red Sea. Comparison of sequences from 189 unknown stomatopod larvae to 327 known adults representing 67 taxa in the superfamily Gonodactyloidea revealed 22 distinct larval operational taxonomic units (OTUs). In the Western Pacific, 10 larval OTUs were members of the Gonodactylidae and Protosquillidae where success of positive identification was expected to be 96.5%. However, only five OTUs could be identified to species and at least three OTUs represent new species unknown in their adult form. In the Red Sea where the identification rate was expected to be 75% in the Gonoclactylidae, none of four larval OTUs could be identified to species; at least two represent new species unknown in their adult forms. Results indicate that the biodiversity in this well-studied group in the Coral Triangle and Red Sea may be underestimated by a minimum of 50% to more than 150%, suggesting a much greater challenge in lesser-studied groups. Although the DNA barcoding methodology was effective, its overall success was limited due to the newly discovered taxonomic limitations of the reference sequence database, highlighting the importance of synergy between molecular geneticists and taxonomists in understanding and documenting our world's biodiversity, both in marine and terrestrial environments.