Cytokeratin expression profile in gastric carcinomas
Cytokeratin expression profile in gastric carcinomas
复制标题
DOI：
10.1016/j.humpath.2003.12.007
复制
发表时间：
2004-05-01
期刊：
影响因子：
3.3
通讯作者：
Kim, WH
中科院分区：
文献类型：
--
作者：
Kim, MA;Lee, HS;Kim, WH
细胞角蛋白（CK）主要在上皮细胞中观察到，CK亚型的不同表达模式可能有助于区分各种器官（包括胃）的原发性和转移性癌。为明确胃癌组织中CK的表达谱，采用组织芯片法对329例胃癌标本进行了CK亚型的免疫组化分析。CK 4、CK 5、CK 6、CK 7、CK 8、CK 10、CK 13、CK 14、CK 16、CK 17、CK 18、CK 19的阳性率分别为84.7%、3.2%、28.7%、71.1%、96.6%、0%、81.6%、0.3%、4.1%、0%、99.4%、89.7%。CK 20为30.0%。高分化或中分化癌与几种CK相关，粘液癌与CK 20表达相关。有趣的是，CK 7和CK 20表达与胃癌的粘蛋白表型相关，但这种关联没有诊断价值。EB病毒（EBV）相关胃癌呈CK 7阴性表达的趋势。CK 6表达与微卫星不稳定性（MSI）、pTNM分期早、临床预后好有关。总之，连续胃癌的CK表达谱表现出异质性，没有单一的CK本身具有诊断价值。CK表达与各种临床病理参数、粘蛋白表型、EBV阳性、MSI状态和患者生存相关。第35章576-581. (C)2004爱思唯尔公司All rights reserved.
Cytokeratin (CK) is observed mainly in epithelial cells, and the diverse expression pattern of CK subtypes may be helpful in discriminating between primary and metastatic carcinomas in various organs, including the stomach. To clarify the CK expression profiles in gastric carcinomas, 329 consecutive specimens were immunohistochemically evaluated in terms of their CK subtypes using the tissue array method. The overall positive rates were 84.7% for CK4, 3.2% for CK5, 28.7% for CK6, 71.1% for CK7, 96.6% for CK8, 0% for CK10, 81.6% for CK13, 0.3% for CK14, 4.1% for CK16, 0% for CK17, 99.4% for CK18, 89.7% for CK19, and 30.0% for CK20. Well-differentiated or moderately differentiated carcinomas were related to several CKs, and mucinous carcinoma was associated with CK20 expression. Interestingly, CK7 and CK20 expression was associated with the mucin phenotype of gastric carcinoma, but this association had no diagnostic value. Epstein-Barr virus (EBV)-associated gastric carcinomas showed a tendency toward negative expression of CK7. CK6 expression was related to microsatellite instability (MSI), early pTNM stage, and better clinical outcome. In conclusion, CK expression profiles in consecutive gastric carcinomas demonstrate heterogeneity, and no single CK has diagnostic value by itself. CK expression is associated with various clinicopathologic parameters, mucin phenotype, EBV positivity, MSI status, and patient survival. Hum PATHOL 35:576-581. (C) 2004 Elsevier Inc. All rights reserved.