Peripheral blood progenitor cells mobilized by recombinant human granulocyte colony-stimulating factor plus recombinant rat stem cell factor contain long-term engrafting cells capable of cellular proliferation for more than two years as shown by serial transplantation in mice.
Peripheral blood progenitor cells mobilized by recombinant human granulocyte colony-stimulating factor plus recombinant rat stem cell factor contain long-term engrafting cells capable of cellular proliferation for more than two years as shown by serial transplantation in mice.
复制标题
重组人粒细胞集落刺激因子加上重组大鼠干细胞因子动员的外周血祖细胞含有能够细胞增殖两年以上的长期移植细胞，如小鼠连续移植所示。
作者：
X. Q. Yan;C. Hartley;P. McElroy;A. Chang;Clay McCrea;I. Mcniece
动员外周血祖细胞（PBPC）已被证明在接受大剂量化疗的患者中提供快速植入。动物模型显示，PBPC含有具有长期移植潜力的细胞。在本研究中，我们进一步分析了动员PBPC支持辐照小鼠连续移植的能力。重组人粒细胞集落刺激因子（rhG-CSF）和重组大鼠干细胞因子（rrSCF）动员PBPC的移植在移植后12至14个月导致98%的原代受体移植。从这些初级受体中获取骨髓（BM）细胞并移植到次级受体中。移植后6个月，所有存活的二次受者都进行了供体移植。聚合酶链反应（PCR）结果显示，脾脏、胸腺和淋巴结中男性细胞占90%以上。继发性受体骨髓细胞的髓系集落在所有动物中均显示雄性来源的粒细胞/巨噬细胞集落形成细胞（GM-CFC）。相比之下，rhG-CSF动员PBPC移植导致继发受体男性移植减少。来自第二次受体的BM细胞，最初接受了由rrSCF和rhG-CSF联合动员的PBPC，进一步传代到第三代女性受体。在移植后6个月，通过全血PCR分析，90%的动物有雄性来源的造血。这些数据表明，用rhG-CSF + rrSCF动员的PBPC含有可移植且能够维持造血功能超过26个月的细胞，这表明动员的长期重构干细胞（LTRC）具有广泛的增殖潜力，类似于BM中的细胞。此外，数据显示，与单独使用rhG-CSF相比，rhG-CSF加rrSCF可增加LTRC的动员。
Mobilized peripheral blood progenitor cells (PBPC) have been shown to provide rapid engraftment in patients given high-dose chemotherapy. PBPC contain cells with long-term engraftment potential as shown in animal models. In this study we have further analyzed mobilized PBPC for their ability to support serial transplantation of irradiated mice. Transplantation of recombinant human granulocyte colony-stimulating factor (rhG-CSF) plus recombinant rat stem cell factor (rrSCF) mobilized PBPC resulted in 98% donor engraftment of primary recipients at 12 to 14 months post-transplantation. Bone marrow (BM) cells from these primary recipients were harvested and transplanted into secondary recipients. At 6 months posttransplantation, all surviving secondary recipients had donor engraftment. Polymerase chain reaction (PCR) analysis showed greater than 90% male cells in spleens, thymuses, and lymph nodes. Myeloid colonies from BM cells of secondary recipients demonstrated granulocyte/macrophage colony-forming cells (GM-CFC) of male origin in all animals. In comparison, transplantation of rhG-CSF mobilized PBPC resulted in decreased male engraftment in secondary recipients. BM cells from secondary recipients, who originally received PBPC mobilized by the combination of rrSCF and rhG-CSF, were further passaged to tertiary female recipients. At 6 months posttransplantation, 90% of animals had male-derived hematopoiesis by whole-blood PCR analysis. These data showed that PBPC mobilized with rhG-CSF plus rrSCF contained cells that are transplantable and able to maintain hematopoiesis for more than 26 months, suggesting that the mobilized long-term reconstituting stem cells (LTRC) have extensive proliferative potential and resemble those that reside in the BM. In addition, the data demonstrated increased mobilization of LTRC with rhG-CSF plus rrSCF compared to rhG-CSF alone.
自我更新正常或减弱的多能干细胞在致命辐射下仍能存活。
影响因子：
2.6
作者：
Brecher,G;Neben,S;Yee,M;Bullis,J;Cronkite,EP
通讯作者：
Cronkite,EP
影响因子：
56.9
作者：
SOUZA, LM;BOONE, TC;WELTE, K
通讯作者：
WELTE, K