Differential immuno-kinetic assays of allergen-specific binding for peanut allergy serum analysis.
Differential immuno-kinetic assays of allergen-specific binding for peanut allergy serum analysis.
复制标题
用于花生过敏血清分析的过敏原特异性结合的差异免疫动力学测定。
DOI：
10.1007/s00216-012-6390-z
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发表时间：
2012
影响因子：
4.3
通讯作者：
Olkhov RV
中科院分区：
文献类型：
--
作者：
Olkhov RV
无标记纳米颗粒阵列平台已被用于检测来自患有花生过敏症的患者的全血清的总花生过敏原特异性结合。从10名患者的血清进行了筛选，对猫和狗皮屑，尘螨和花生过敏原蛋白Ara H1的四过敏原面板。使用两种二抗IgG和IgE特异性抗体鉴定了IgE和IgG对Ara h1总特异性结合蛋白载量的贡献，发现其贡献小于总特异性蛋白载量的50%。IgE、IgE和Ara h1的未分辨特异性结合蛋白ΔsBP的总质量为高RAST等级患者5和6提供了新的血清谱，IgG/IgE比为4 ± 2，ΔsBP/IgE比为17 ± 11，这两个比值对小型患者队列均无保护作用。图特异性IgE/IgG和未分辨材料X与过敏原功能化的传感器表面的结合
A label-free nanoparticle array platform has been used to detect total peanut allergen-specific binding from whole serum of patients suffering from peanut allergy. The serum from 10 patients was screened against a four-allergen panel of cat and dog dander, dust mite and peanut allergen protein Ara h1. The IgE and IgG contributions to the total specific-binding protein load to Ara h1 were identified using two secondary IgG- and IgE-specific antibodies and were found to contribute less than 50 % of the total specific protein load. The total mass of IgE, IgE and the unresolved specific-binding protein ΔsBP for Ara h1 provides a new serum profile for high-RAST-grade patients 5 and 6 with the IgG/IgE ratio of 4 ± 2 and ΔsBP/IgE ratio of 17 ± 11, neither of which is protective for the small patient cohort.FigureBinding of the specefic IgE/IgG and unresolved material X to the sensor surface functionalized with allergen