Flavipin in Chaetomium globosum CDW7, an endophytic fungus from Ginkgo biloba, contributes to antioxidant activity
Flavipin in Chaetomium globosum CDW7, an endophytic fungus from Ginkgo biloba, contributes to antioxidant activity
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DOI：
10.1007/s00253-013-5013-8
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发表时间：
2013-08-01
影响因子：
5
通讯作者：
Liu, Junyan
中科院分区：
文献类型：
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作者：
Ye, Yonghao;Xiao, Yu;Liu, Junyan
1,2-苯二甲醛-3,4,5-三羟基-6-甲基（flavipin）被发现对线虫和真菌具有拮抗作用。在这里，我们证明黄维平在体外和体内都是一种有效的抗氧化剂，在治疗自由基相关疾病方面具有巨大的潜力。因此，从银杏叶中的80种内生真菌中筛选出产黄素的生物源。 7种内生真菌能够合成抗氧化物质，并通过ITS rDNA序列进行鉴定。其中，Chaetomium globosum CDW7是黄维平的重要生产者。然后优化 CDW7 的发酵参数以实现高黄维素产量。在最佳条件（25℃，100/250mL烧瓶，12盘/烧瓶，150rpm，pH 6.5）下培养14天，CDW7能够以315.5mg/L的产量合成黄维平。此外，黄维平产量与粗提物的抗氧化活性呈正相关，相关系数为0.8235，表明黄维平是CDW7代谢物的主要抗氧化成分。这些数据表明 CDW7 是抗氧化剂黄维平的高产生物来源。
1,2-Benzenedicarboxaldehyde-3,4,5-trihydroxy-6-methyl (flavipin) was found to be antagonistic against nematodes and fungi. Here we demonstrated that flavipin is a potent antioxidant in vitro and in vivo, which has great potential in the therapy for free radical-associated diseases. Therefore, flavipin-producing bio-source was screened from 80 endophytes in Ginkgo biloba. Seven endophytic fungi were able to synthesize antioxidant substances and identified by ITS rDNA sequences. Among them, Chaetomium globosum CDW7 was a remarkable producer of flavipin. The fermentation parameters of CDW7 were then optimized for high flavipin production. Cultured under the optimal condition (25 A degrees C, 100/250 mL flask, 12 discs/flask, 150 rpm, pH 6.5) for 14 days, CDW7 was able to synthesize flavipin at a production of 315.5 mg/L. In addition, flavipin output was positively correlated to antioxidant activities of crude extracts with a correlation coefficient of 0.8235, indicating that flavipin was the major antioxidant component of CDW7's metabolites. These data demonstrated that CDW7 was a highly yielded bio-source of antioxidant flavipin.