Dibutyryl cGMP raises cytosolic concentrations of Ca2+ in cultured nodose ganglion neurons of the rabbit.
Dibutyryl cGMP raises cytosolic concentrations of Ca2+ in cultured nodose ganglion neurons of the rabbit.
复制标题
二丁酰 cGMP 会提高培养的兔子结状神经节神经元中 Ca2+ 的胞质浓度。
DOI：
10.1016/s0006-8993(98)01021-x
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发表时间：
1998
期刊：
影响因子：
2.9
通讯作者：
Kawatani,M
中科院分区：
文献类型：
--
作者：
Sato,M;Kawatani,M
The effect of dibutyryl cGMP (dbcGMP), a membrane permeant cGMP analogue, on cytosolic concentrations of Ca2+([Ca2+]i) was studied in cultured nodose ganglion neurons of the rabbit using fura-2AM and microfluorometry. Application of dbcGMP (10–1000 μM) increased [Ca2+]iin 42% of neurons (n=67). The effect was observed in a dose-dependent fashion. The threshold dose was 100 μM and the increase at 500 μM averaged 117±8%. Removal of extracellular Ca2+abolished the dbcGMP effect. Application of Ni2+(1 mM) or neomycin (50 μM), a non-L-type voltage-gated Ca2+channel (VGCC) antagonist, eliminated the dbcGMP effect. ω-conotoxin GVIA (2 μM), the N-type Ca2+channel antagonist, or L-type Ca2+channel antagonists (D600, 50 μM, or nifedipine, 10 μM) did not alter the dbcGMP effect. Ryanodine (10 μM) did not alter the effect of dbcGMP. Therefore, cGMP could play a part of role of an intracellular messenger in primary sensory neurons of the autonomic nervous system.
