Luminescent silica nanobeads: Characterization and evaluation as efficient cytoplasmatic transporters for T-lymphocytes
Luminescent silica nanobeads: Characterization and evaluation as efficient cytoplasmatic transporters for T-lymphocytes
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DOI：
10.1021/ja070245c
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发表时间：
2007-06-27
影响因子：
15
通讯作者：
Mustelin, Tomas
中科院分区：
文献类型：
--
作者：
Bottini, Massimo;Cerignoli, Fabio;Mustelin, Tomas
我们报道了掺杂钌配合物发光体且用抗人CD3、抗人CD28以及一种酸敏感聚合物进行功能化的中性抗生物素蛋白共轭二氧化硅纳米珠的制备及特性。我们观察到，纳米珠通过内吞作用很容易被递送到Jurkat T白血病细胞中，被转运到溶酶体，随后如pH敏感发光所显示的那样进入细胞质。由于未观察到细胞毒性迹象，所报道的纳米珠可能是一种用于高效细胞内转运的优良且无毒的构建模块。
We report the fabrication and characterization of neutravidin-conjugated silica nanobeads doped with a ruthenium-complex luminophore and functionalized with antihuman CD3, antihuman CD28, and an acid-sensitive polymer. We observed that the nanobeads were readily delivered into Jurkat T leukemia cells by endocytosis, transported into lysosomes and subsequently into the cytoplasm as revealed by pH-sensitive luminescence. Since signs of cytotoxicity were not observed, the reported nanobeads could be an excellent and nontoxic building block for efficient intracellular transporters.