Expression of the Ubiquitin Ligase HRD1 in Neural Stem/Progenitor Cells of the Adult Mouse Brain
Expression of the Ubiquitin Ligase HRD1 in Neural Stem/Progenitor Cells of the Adult Mouse Brain
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DOI：
10.1254/jphs.11120sc
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发表时间：
2011-11-01
影响因子：
3.5
通讯作者：
Okuma, Yasunobu
中科院分区：
文献类型：
--
作者：
Kawada, Koichi;Kaneko, Masayuki;Okuma, Yasunobu
神经干细胞(NSCs)位于成年哺乳动物海马齿状回的脑室下区(SVZ)和颗粒下区。泛素连接酶Hrd1与淀粉样前体蛋白的降解有关，据信在神经元中特异表达，而不是在星形胶质细胞中表达。我们用免疫组织化学方法研究了Hrd1的表达，发现Hrd1与NSC标志蛋白Nestin和胶质纤维酸性蛋白共存于SVZ(SVZ星形胶质细胞)的NSCs中，而不是在海马区。在海马齿状回，Hrd1定位于巢蛋白阳性细胞的胞核。
Neural stem/progenitor cells (NSCs) reside in the subventricular zone (SVZ) and subgranular zone of the hippocampal dentate gyrus in adult mammals. The ubiquitin ligase HRD1 is associated with degradation of amyloid precursor protein and believed to be specifically expressed in neurons and not in astrocytes. We investigated expression of HRD1 using immunohistochemistry and found colocalization of HRD1 with the NSC marker protein nestin and glial fibrillary acidic protein in the NSCs of the SVZ (the SVZ astrocytes) but not in the hippocampus. In the hippocampal dentate gyrus, HRD1 is localized in the nucleus of nestin-positive cells.