Micro-LED Array-Based Photo-Stimulation Devices for Optogenetics in Rat and Macaque Monkey Brains
Micro-LED Array-Based Photo-Stimulation Devices for Optogenetics in Rat and Macaque Monkey Brains
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DOI：
10.1109/access.2021.3111666
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发表时间：
2021-01-01
期刊：
影响因子：
3.9
通讯作者：
Ohta, Jun
中科院分区：
文献类型：
--
作者：
Ohta, Yasumi;Guinto, Mark Christian;Ohta, Jun
光遗传学是利用光控制生物功能的有力工具。光纤已广泛应用于光遗传学的光学刺激装置中。然而，光纤的使用导致小的光刺激区域。在这项研究中，开发了微型LED阵列设备，以实现大型动物（如猕猴）大脑中的大面积光刺激。设计并制造了平面和线性微LED阵列器件，以光刺激大脑的前额叶皮层（PFC）和腹侧被盖区（VTA）并诱导神经化学反应。首先使用大鼠表征器械操作、光强度和安全性。随后，这些设备被用来光刺激猕猴的大脑。此外，在PFC中进行微透析。这些设备检测到大脑中多巴胺的调节水平。因此，成功地实现了PFC和VTA的光刺激，并且证明了所开发的微LED阵列器件的有效性。该研究将有助于促进对微型LED阵列刺激的进一步研究，用于大型动物的全系统光遗传学操作。
Optogenetics is a powerful tool for controlling biological functions using light. Optical fibers have been extensively utilized in optical stimulation devices for optogenetics. However, the use of optical fibers results in a small photo-stimulation region. In this study, micro-LED array devices were developed to achieve large-area photo-stimulation in the brain of a large animal, such as macaque monkeys. Planar and linear micro-LED array devices were designed and fabricated to photo-stimulate the prefrontal cortex (PFC) and ventral tegmental area (VTA) of the brain and induce a neurochemical response. Device operation, optical intensity, and safety were first characterized using rats. Subsequently, the devices were used to photo-stimulate the brain of macaque monkeys. In addition, microdialysis in the PFC was performed. The devices detected modulated levels of dopamine in the brains. Thus, the photo-stimulation of both the PFC and VTA were successfully achieved, and the effectiveness of the developed micro-LED array devices was demonstrated. The study will help facilitate further studies on micro-LED array stimulation for system-wide optogenetic manipulation in large animals.