Astrocytes protect against X-ray-induced neuronal toxicity in vitro.
Astrocytes protect against X-ray-induced neuronal toxicity in vitro.
复制标题
星形胶质细胞在体外可防止 X 射线诱导的神经元毒性。
DOI：
10.1097/00001756-199804200-00032
复制
发表时间：
1998
期刊：
影响因子：
1.7
通讯作者：
Tofilon,PJ
中科院分区：
文献类型：
--
作者：
Noel,F;Tofilon,PJ
神经元损伤在脑肿瘤治疗引起的放射诱导的CNS损伤中的作用仍然知之甚少。为了开始定义神经元的放射反应，研究了大鼠皮层神经元培养物的存活。3.5戈伊照射后24-48 h，神经元存活率下降约40%。照射后加入自由基清除剂NAC增加了神经元的存活。在与星形胶质细胞共培养或在星形胶质细胞条件培养基存在下，神经元对辐射的敏感性也显著降低。发现星形胶质细胞条件培养基获得显著的自由基清除能力。然而，这种抗氧化特性似乎并不负责神经元的辐射保护。星形胶质细胞减少辐射诱导的神经元毒性的能力似乎是由mol. wt> 10 kDa。
THE role of neuronal damage in the radiation-induced CNS injury resulting from brain tumor therapy remains poorly understood. To begin to define the radioresponse of neurons, the survival of rat cortical neuron cultures was investigated. Neuronal survival was reduced by∼ 40% 24–48 h after irradiation with 3.5 Gy. The addition of the free radical scavenger NAC after irradiation increased neuronal survival. Neurons were also significantly less sensitive to radiation in co-cultures with astrocytes or in the presence of astrocyte-conditioned medium. Medium conditioned on astrocytes was found to acquire significant free radical scavenging capability. However, this antioxidant property does not appear to be responsible for neuronal radioprotection. The ability of astrocytes to reduce radiation-induced neuronal toxicity appears to be mediated by a soluble protein (s) of mol. wt> 10 kDa.