Glucocorticoid and progesterone receptors bind to the same sites in two hormonally regulated promoters
Glucocorticoid and progesterone receptors bind to the same sites in two hormonally regulated promoters
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糖皮质激素和孕激素受体结合到两个激素调节启动子的相同位点
作者：
D. V. D. Ahe;S. Janich;C. Scheidereit;R. Renkawitz;G. Schütz;M. Beato
大鼠肝脏的糖皮质激素受体识别基因转移实验中激素诱导所需的小鼠乳腺肿瘤病毒（MMTV）启动子附近的核苷酸序列1 -4。在人金属硫蛋白基因IIA（参考文献5）和鸡溶菌酶基因6中发现了类似的核苷酸序列，后者也由雌激素、孕酮和雄激素诱导。在显微注射实验中，溶菌酶启动子的44个碱基对（bp）（从-208到-164）的缺失导致孕酮和糖皮质激素依赖性基因表达的协调损失。我们在这里表明，纯化的糖皮质激素受体从大鼠肝脏和孕激素受体从兔子宫产生类似或重叠的外切核酸酶III的足迹在MMTV和鸡溶菌酶的启动子区域。因此，不同类固醇激素的调节元件可以是相似的或至少共享结构特征。
The glucocorticoid receptor of rat liver recognizes nucleotide sequences near the promoter of mouse mammary tumour virus (MMTV) required for hormonal induction in gene transfer experiments1–4. Similar nucleotide sequences have been found in the human metallothionein gene IIA (ref. 5) and in the chicken lysozyme gene6, the latter induced also by oestrogen, progesterone and androgens. In microinjection experiments, deletion of only 44 base pairs (bp) of the lysozyme promoter (from −208 to −164) results in coordinated loss of progesterone and glucocorticoid-dependent gene expression6. We show here that purified glucocorticoid receptor from rat liver and progesterone receptor from rabbit uterus yield similar or overlapping exonuclease III footprints in the promoter regions of MMTV and chicken lysozyme. Thus, the regulatory elements for different steroid hormones may be similar or at least share structural features.
雌激素初次刺激期间雏鸡输卵管激素反应性的变化。
影响因子：
2.7
作者：
Moen,RC;Palmiter,RD
通讯作者：
Palmiter,RD