Isolation of high-molecular-weight DNA from eukaryotic cells by formamide treatment and dialysis.
Isolation of high-molecular-weight DNA from eukaryotic cells by formamide treatment and dialysis.
复制标题
通过甲酰胺处理和透析从真核细胞中分离高分子量 DNA。
DOI：
10.1016/0003-2697(87)90366-6
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发表时间：
1987
影响因子：
2.9
通讯作者：
R. Emanoil
中科院分区：
文献类型：
--
作者：
J. Kupiec;M. Giron;D. Vilette;J. Jeltsch;R. Emanoil
提出了一种从真核细胞中提取高分子量DNA的新方法。它是基于甲酰胺处理和细胞蛋白酶K酶的广泛透析。该程序主要包括一系列孵育，允许同时制备几种DNA，然后可以限制、连接、杂交和克隆。
A new method for the isolation of high-molecular-weight DNA from eukaryotic cells is presented. It is based on formamide treatment and extensive dialysis of cellular proteinase K digests. This procedure consists mainly of a series of incubations allowing simultaneous preparation of several DNAs which can then be restricted, ligated, hybridized, and cloned.