Deletion of a cyclin-dependent protein kinase inhibitor, CsSMR1, leads to dwarf and determinate growth in cucumber (Cucumis sativus L.).
Deletion of a cyclin-dependent protein kinase inhibitor, CsSMR1, leads to dwarf and determinate growth in cucumber (Cucumis sativus L.).
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DOI：
10.1007/s00122-021-04006-7
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Li S;Zhang Q;Zhang H;Wang J;Sun J;Yang X;Huang S;Zhang Z
一个含有周期蛋白依赖性蛋白激酶抑制剂的7.9 kb缺失导致黄瓜生长和矮化表型的确定。植物的构型是一个复合性状，主要由茎分枝、节间伸长和茎的决定论所决定。理想的建筑往往会增加植物的产量，就像20世纪60年代应用半矮秆谷物作物品种增加的“绿色革命”一样。黄瓜（Cucumis sativus L.）是世界上重要的蔬菜种植品种，在不同的生产体系中选择了合适的建筑品种。在这项研究中，我们获得了一个新的矮秆突变体，其株高明显缩短，生长习性确定。通过大量分离分析和图谱克隆，我们将dw2基因座划分为56.4 kb的区域，包含5个基因。在WT和dw2之间56.4 kb区域的所有变异中，导致dw2中CsaV3_5G035790基因完全缺失的7.9 kb缺失与矮化表型共分离。单倍型分析和基因表达分析表明，编码周期蛋白依赖性蛋白激酶抑制剂（CsSMR1）的CsaV3_5G035790是dw2矮化表型的候选基因。RNA-seq分析显示，在WT和dw2中，一些激酶样蛋白、细胞周期蛋白和器官大小调节因子的表达存在差异，这可能是矮秆植物器官大小减小的原因。另外，在dw2阶段，CsSTM和CsWOX9基因表达下调，导致茎尖分生组织发育过早终止，最终导致节间数减少，株高降低。CsSMR1基因的鉴定和鉴定为黄瓜结构改造在机械化生产系统中的应用提供了新的思路。在线版本包含补充材料，可在10.1007/s00122-021-04006-7获得。
A 7.9 kb deletion which contains a cyclin-dependent protein kinase inhibitor leads to determinate growth and dwarf phenotype in cucumber. Plant architecture is a composite character which are mainly defined by shoot branching, internode elongation and shoot determinacy. Ideal architecture tends to increase the yield of plants, just like the case of “Green Revolution” increased by the application of semi-dwarf cereal crop varieties in 1960s. Cucumber (Cucumis sativus L.) is an important vegetable cultivated worldwide, and suitable architecture varieties were selected for different production systems. In this study, we obtained a novel dwarf mutant with strikingly shortened plant height and determinate growth habit. By bulked segregant analysis and map-based cloning, we delimited the dw2 locus to a 56.4 kb region which contain five genes. Among all the variations between WT and dw2 within the 56.4 kb region, a 7.9 kb deletion which resulted in complete deletion of CsaV3_5G035790 in dw2 was co-segregated with the dwarf phenotype. Haplotype analysis and gene expression analysis suggest that CsaV3_5G035790 encoding a cyclin-dependent protein kinase inhibitor (CsSMR1) be the candidate gene responsible for the dwarf phenotype in dw2. RNA-seq analysis shows that several kinesin-like proteins, cyclins and reported organ size regulators are expressed differentially between WT and dw2, which may account for the reduced organ size in dwarf plants. Additionally, the down-regulation of CsSTM and CsWOX9 in dw2 resulted in premature termination of shoot apical meristem development, which eventually reduces the internode number and plant height. Identification and characterization of the CsSMR1 provide a new insight into cucumber architecture modification to be applied to mechanized production system. The online version contains supplementary material available at 10.1007/s00122-021-04006-7.
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