Investigation of the component reactions of oxidative demethylation of sterols. Metabolism of 4 -hydroxymethyl steroids.
Investigation of the component reactions of oxidative demethylation of sterols. Metabolism of 4 -hydroxymethyl steroids.
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甾醇氧化脱甲基化组分反应的研究。
DOI：
10.1016/s0021-9258(18)61979-9
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
J. Gaylor
中科院分区：
文献类型：
--
作者：
W. L. Miller;D. R. Brady;J. Gaylor
本文研究了4α-羟甲基甾醇在大鼠肝微粒体中的代谢。4α-羟甲基氧化成羧酸需要氧和还原的吡啶核苷酸。另一方面，厌氧脱氢生成羧酸非常缓慢。因此，与早期的建议形成鲜明对比，羟甲基的氧化似乎是由微粒体混合功能氧化酶催化的。4α-羟甲基-5 α-胆甾-7-烯-3 β-醇和4β-甲基-4 α-羟甲基-5 α-胆甾-7-烯-3 β-醇的氧化可能由同一肝微粒体系统催化。此外，催化由4α-甲基和4α-羟甲基形成4α-羧酸的氧化酶的性质非常相似。
The metabolism of 4α-hydroxymethyl sterols by rat liver microsomes has been studied. Oxidation of the 4α-hydroxymethyl group to a carboxylic acid requires oxygen and reduced pyridine nucleotide. On the other hand, anaerobic dehydrogenation to the carboxylic acid is very slow. Thus, in sharp contrast to earlier suggestions, it appears that oxidation of the hydroxymethyl group is catalyzed by microsomal mixed function oxidases. Oxidations of 4α-hydroxymethyl-5α-cholest-7-en-3β-ol and 4β-methyl-4α-hydroxymethyl-5α-cholest-7-en-3β-ol may be catalyzed by the same liver microsomal system. Furthermore, the properties of the oxidases that catalyze formation of 4α-carboxylic acids from 4α-methyl and 4α-hydroxymethyl groups are very similar.