A multiplex PCR assay for molecular sexing of the naked mole-rat (Heterocephalus glaber)
A multiplex PCR assay for molecular sexing of the naked mole-rat (Heterocephalus glaber)
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DOI：
10.1111/j.1755-0998.2009.02742.x
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发表时间：
2010-01-01
影响因子：
7.7
通讯作者：
Matsuda, Y.
中科院分区：
文献类型：
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作者：
Katsushima, K.;Nishida, C.;Matsuda, Y.
为了对裸鼹鼠（Heterocephalus glaber）进行分子性别鉴定，我们设计了一套PCR引物，扩增了Y连锁的DBY基因的一部分。将该引物组应用于已知性别的样品，以16SrRNA基因（16SrDNA）引物作为对照，成功地获得了雄性的两条DNA带，雄性特有的163bp DBY带和雌性共有的446bp 16SrDNA带，而雌性仅显示共有带。结果表明，该方法可用于H. glaber。
For molecular sexing of the naked mole-rat (Heterocephalus glaber), we designed a PCR primer set to amplify part of the Y-linked DBY gene. When this primer set was applied to the samples of known sex with the 16S rRNA gene (16S rDNA) primers as control, PCR products were successfully obtained as two DNA bands in males, a male-specific 163 bp DBY band and a 446 bp band of 16S rDNA shared with females, whereas females showed only the common band. This result shows that this multiplex PCR assay is useful for sex identification of H. glaber.