Determination of dansylated monoamine and amino acid neurotransmitters and their metabolites in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry
Determination of dansylated monoamine and amino acid neurotransmitters and their metabolites in human plasma by liquid chromatography-electrospray ionization tandem mass spectrometry
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DOI：
10.1016/j.ab.2009.09.015
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Li, Huan-De
中科院分区：
文献类型：
--
作者：
Cai, Hua-Lin;Zhu, Rong-Hua;Li, Huan-De
神经递质（NTs）及其代谢产物的测定有助于更好地理解中枢神经系统疾病中复杂的神经生物学，并在许多其他领域具有广泛的用途。建立了液相色谱-电喷雾串联质谱法用于定量多巴胺（DA）、3，4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）、去甲肾上腺素（NE）、香草扁桃酸（VMA）、3-甲氧基-4-羟基苯乙二醇（MHPG）、5-羟色胺（5-HT）、5-羟基吲哚-3-乙酸（5-HIAA）、谷氨酸（Glu）、和γ-氨基丁酸（GABA）。将NT及其代谢物丹磺酰化，并通过LC梯度在C18柱上用串联质谱仪在线分析。该方法对所有分析物均表现出良好的线性，回归系数大于0.99。DA、DOPAC、HVA、NE、VMA、MHPG、5-HT、5-HIAA、Glu和GABA的定量下限（LLOQ）值分别为0.57、0.37、0.35、0.40、0.35、0.91、0.27、0.43、0.65和1.62 pmol/ml。精密度结果表示为变异系数（CV），批内范围为1.5%-13.6%，批间范围为2.9%-13.7%。这种新的LC-ESI/MS/MS方法具有精确、高灵敏度、特异性和足够简单的特点。它可以提供一种替代的方法来定量的NT及其代谢产物在人血浆中。(C)2009 Elsevier Inc. All rights reserved.
The determination of neurotransmitters (NTs) and their metabolites facilitates better understanding of complex neurobiology in the central nervous system disorders and has expanding uses in many other fields. We present a liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS) method for the quantification of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), norepinephrine (NE), vanillymandelic acid (VMA), 3-methoxy-4-hydroxy phenylglycol (MHPG), 5-hydroxytryptamine (5-HT), 5-hydroxyindole-3-acetic acid (5-HIAA), glutamate (Glu), and gamma-aminobutyric acid (GABA). The NTs and their metabolites were dansylated and analyzed by an LC gradient on a C18 column on-line with a tandem mass spectrometer. This method exhibited excellent linearity for all of the analytes with regression coefficients higher than 0.99. The lower limit of quantification (LLOQ) values for DA, DOPAC, HVA, NE, VMA, MHPG, 5-HT, 5-HIAA, Glu, and GABA were 0.57, 0.37, 0.35, 0.40, 0.35, 0.91, 0.27, 0.43, 0.65, and 1.62 pmol/ml, respectively. The precision results were expressed as coefficients of variation (CVs), ranging from 1.5% to 13.6% for intraassay and from 2.9% to 13.7% for the interassay. This novel LC-ESI/MS/MS approach is precise, highly sensitive, specific, and sufficiently simple. It can provide an alternative method for the quantification of the NTs and their metabolites in human plasma. (C) 2009 Elsevier Inc. All rights reserved.