Simple method for visualization of the islets in fixed but otherwise intact pancreas.
Simple method for visualization of the islets in fixed but otherwise intact pancreas.
复制标题
用于可视化固定但完整的胰腺中的胰岛的简单方法。
作者：
K. G. Kramlinger;R. Mayrand;N. Lifson
一个简单的，廉价的，可重复的方法描述突出显示胰岛。该方法包括用生理盐水、福尔马林、苏木精和水对处死动物的器官进行顺序动脉灌注，然后在水杨酸甲酯中清洗。当需要定量区分胰岛组织和非胰岛组织，并允许固定时，该方法是有用的。
A simple, inexpensive, reproducible method is described for prominently displaying the islets of Langerhans. The method consists of sequential arterial perfusion of the organ of the sacrificed animal with saline, formalin, hematoxylin, and water, followed by clearing in methyl salicylate. The procedure should be useful whenever islet tissue needs to be quantitatively distinquished from non islet tissue and fixation is allowable.