Separation of Agar Colony Forming Cells from Rat Bone Marrow 1
Separation of Agar Colony Forming Cells from Rat Bone Marrow 1
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从大鼠骨髓中分离琼脂集落形成细胞 1
DOI：
10.3181/00379727-145-37843
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发表时间：
1974
影响因子：
--
通讯作者：
A. S. Gordon
中科院分区：
文献类型：
--
作者：
J. R. Zucali;Frederic Rakowitz;G. van Zant;A. S. Gordon
摘要 大鼠骨髓细胞经注射内毒素的大鼠获得的集落刺激因子 (CSF) 刺激后，在琼脂中形成集落。在不连续的牛血清白蛋白密度梯度（BSA）上进行大鼠琼脂集落形成细胞（CFC）的分离。当与 CSF 一起培养时，获得的 26.5% 至 29.0% BSA 之间的细胞级分显示出大鼠琼脂集落形成细胞的富集。尽管细胞分离尚未完成，但目前的研究支持这样的论点：介于小淋巴细胞和“母细胞”之间且类似于“过渡细胞”的细胞亚群负责产生琼脂集落。
Summary Rat bone marrow cells formed colonies in agar after stimulation by colony stimulating factor (CSF) obtained from endotoxin-injected rats. Separation of rat agar colony forming cells (CFCs) was performed on a discontinuous bovine serum albumin density gradient (BSA). Cell fractions obtained between 26.5 and 29.0% BSA showed enrichment for rat agar colony forming cells when cultured with CSF. Although the cell separation has not been complete the present studies support the contention that a subpopulation of cells intermediate between small lymphocytes and “blast” cells and resembling “transitional cells” are responsible for the agar colonies produced.