Establishment and application of PCR-SSP for detecting Mur blood group
Establishment and application of PCR-SSP for detecting Mur blood group
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发表时间：
2009
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通讯作者：
L. Yong
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作者：
L. Yong
目的建立Mur血型的分子检测方法。方法根据Mur血型的分子背景设计Mur基因序列特异性引物，建立Mur血型检测的PCR-SSP方法。对PCR反应条件进行了优化。该方法经标准样品验证，并应用于献血者Mur血型检测。结果建立了Mur血型分子检测方法。并通过标准样品验证了该方法的有效性(P0.01)。通过该方法和血清学检测，共对107名随机献血者进行了平行Mur血型检测。该方法结果与血清学检测结果差异无统计学意义(P<0。01)。结论 Mur血型分子检测方法可用于Mur血型的检测。
Objective To establish the molecular detection method of Mur blood group.Methods To design Mur gene sequence specific primers according to the molecular background of Mur blood group and establish the PCR-SSP to detect Mur blood group. The conditions of PCR reaction was optimized. This method was validated by standard samples and applied in Mur blood group detection of blood donors.Results The molecular method of Mur blood group was established. And this methods was validated by standard samples(P0.01). A total of 107 random donors were detected parallel Mur blood group by this method and serology test. The results of this method showed that there was no statistically significant difference with serology test(P0.01). Conclusion The molecular method of Mur blood group can be applied in detection of Mur blood group.